Monensin, a specific sodium ionophore, has been shown to reduce glucose-induced proinsulin biosynthesis by 30% and to completely inhibit the intracellular conversion of proinsulin to insulin. Autoradiography of monensin-treated cells demonstrated the presence of large quantities of newly synthesized proteins in amorphous vesicles close to the Golgi complex of B ceils. The results suggest profound effects of monensin on biosynthesis and intracellular processing of proinsulin.

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