Amoebae of the cellular slime mould Dictyostelium discoideum at 8 h or 10 h development were separated into two populations by countercurrent distribution in a dextran-poly(ethylene glycol), two-phase system. Two-dimensional, polyacrylamide-gel electrophoresis was then used to separate the polypeptides from the populations of amoebae. The two populations of amoebae at 8 h development differed sn polypeptide composition as did the populations separated at 10 h development. This confirms that cell differentiation is initated in D. discoideum prior to 8 h development.
Polypeptide compositions of amoebae of the cellular slime mould Dictyostelium discoideum separated by partitioning during development
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P. T. Sharpe, R. M. Sharrard, D. J. Watts; Polypeptide compositions of amoebae of the cellular slime mould Dictyostelium discoideum separated by partitioning during development. Biosci Rep 1 February 1985; 5 (2): 121–127. doi: https://doi.org/10.1007/BF01117058
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