Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [15S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins were transferred electrophoretically on nitrocellulose paper. T antigen was detected on nitrocellulose strips by using for the first time, specific, purified antipeptide monoclonal antibodies directed against the N- and C-terminal portions of the molecule, and125I-labeled Protein A.
Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by western blotting
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Jean Bernard Dietrich; Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by western blotting. Biosci Rep 1 February 1985; 5 (2): 137–142. doi: https://doi.org/10.1007/BF01117060
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