Transcription studies with highly purified potato cell nuclei in combination with a ‘transcription-hybridization analysis’ unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and α-amanitin furthermore suggest that the nuclear DNA-dependent RNA polymerases I and II are involved in the synthesis of PSTV (+) and (−) RNA, respectively.

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