High-affinity binding of [3H]folate to supernatant from homogenized human leukocytes containing large amounts of binding protein displayed apparent positive cooperativity. The DEAE-Sepharose® CL-6B chromatographic profile of the supernatant at pH 6.3 contained a major peak of folate binding (Mr approx. 25 000) in the front effluent and a smaller more acidic peak (Mr approx. 25 000) that emerged after a rise in NaCl from 30 mmol/l to 1 mol/l. Triton X-100 solubilized ceil sediment from the leukocyte homogenate contained some high-affinity folate binding activity (Mr approx 25 000), typically 5–10% of the total binding activity.
A high-affinity folate binding protein in normal human leukocytes: Ligand binding characteristics, ionic charge and molecular size
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Jan Holm, Steen Ingemann Hansen, J⊘rgen Lyngbye; A high-affinity folate binding protein in normal human leukocytes: Ligand binding characteristics, ionic charge and molecular size. Biosci Rep 1 August 1985; 5 (8): 683–688. doi: https://doi.org/10.1007/BF01117000
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