A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%) of PHA (10 μg/ml) or ConA (25 μg/ml) proliferative effects. Thus, the activation of membrane-bound PLC is a sine-qua-non condition for lectin-induced proliferation of T lymphocytes. The key-role of PLC versus protein kinase C (PKC) is stressed by the fact that the inhibition of PKC with Hidaka's compound H-7 (40 μM) produced only a partial blockade (about 25%) of lectin mitogenic effect.
Anti-phospholipase C antibodies inhibit the lectin-induced proliferation of human lymphocytes
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I. I. Moraru, Mioara Manciulea, Anca Cǎlugǎru, Gr. Ghyka, L. M. Popescu; Anti-phospholipase C antibodies inhibit the lectin-induced proliferation of human lymphocytes. Biosci Rep 1 September 1987; 7 (9): 731–736. doi: https://doi.org/10.1007/BF01116866
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