Bioscience Reports (2018)https://doi.org/10.1042/BSR20180231
After acceptance and the publication of the Accepted 3, follow-up experiments revealed unexpected variability in the binding constants obtained by the mixed-KD analysis of FP binding data as shown in Table 2 in the Accepted Manuscript. Further investigation using non-reducing SDS–PAGE found previously undetected PDZ–PDZ dimerization triggered by extended incubation with the small-molecule compounds under stringent labeling conditions. This dimerization is not accounted for by our mixed-KD model. The final Version of Record instead reports apparent KD values determined from FP titrations with PDZ domains treated to minimize PDZ–PDZ dimerization while preserving high levels of small-molecule adduct formation, together with associated changes in text.
This has been approved by the Editorial Board of Bioscience Reports.