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Articles

Biochemical characterization of paralyzed flagellum proteins A (PflA) and B (PflB) from Helicobacter pylori flagellar motor

Xiaotian Zhou, Mohammad F. Khan, Yue Xin, Kar L. Chan, Anna Roujeinikova
Journal: Bioscience Reports
Biosci Rep (2024) 44 (9): BSR20240692.
https://doi.org/10.1042/BSR20240692
Published: 10 September 2024
View articletitled, Biochemical characterization of paralyzed flagellum proteins A (PflA) and B (PflB) from Helicobacter pylori flagellar motor
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Includes: Supplementary data
Articles

Structural characterization of DNA-binding domain of essential mammalian protein TTF 1

Gajender Singh, Abhinetra Jagdish Bhopale, Saloni Khatri, Prashant Prakash, Rajnish Kumar, Sukh Mahendra Singh, Samarendra Kumar Singh
Journal: Bioscience Reports
Biosci Rep (2024) 44 (8): BSR20240800.
https://doi.org/10.1042/BSR20240800
Published: 28 August 2024
View articletitled, Structural characterization of DNA-binding domain of essential mammalian protein TTF 1
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Includes: Supplementary data
Articles

Expression, purification and application of a recombinant, membrane permeating version of the light chain of botulinum toxin B

Micaela Vanina Buzzatto, Fabiana Cristina Benegas Guerrero, Pablo Ariel Álvarez, María Paz Zizzias, Luis Mariano Polo, Claudia Nora Tomes
Journal: Bioscience Reports
Biosci Rep (2024) 44 (7): BSR20240117.
https://doi.org/10.1042/BSR20240117
Published: 30 July 2024
View articletitled, Expression, purification and application of a recombinant, membrane permeating version of the light chain of botulinum toxin B
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Includes: Supplementary data
Articles

Protein disulfide isomerase uses thrombin–antithrombin complex as a template to bind its target protein and alter the blood coagulation rates

Abdul Burhan Khan, Urfi Siddiqui, Sana Fatima, Ahmed Abdur Rehman, Mohamad Aman Jairajpuri
Journal: Bioscience Reports
Biosci Rep (2024) 44 (5): BSR20231540.
https://doi.org/10.1042/BSR20231540
Published: 15 May 2024
View articletitled, Protein disulfide isomerase uses thrombin–antithrombin complex as a template to bind its target protein and alter the blood coagulation rates
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Includes: Supplementary data
Articles

Spectroscopic analysis of the bacterially expressed head domain of rotavirus VP6

Milaan Simone Strachan, Tshepo Mashapa, Samantha Gildenhuys
Journal: Bioscience Reports
Biosci Rep (2024) 44 (5): BSR20232178.
https://doi.org/10.1042/BSR20232178
Published: 29 April 2024
View articletitled, Spectroscopic analysis of the bacterially expressed head domain of rotavirus VP6
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Includes: Supplementary data
Articles

Excitation energy transfer in proteoliposomes reconstituted with LH2 and RC-LH1 complexes from Rhodobacter sphaeroides

Xia Huang, Cvetelin Vasilev, David J.K. Swainsbury, C. Neil Hunter
Journal: Bioscience Reports
Biosci Rep (2024) 44 (2): BSR20231302.
https://doi.org/10.1042/BSR20231302
Published: 19 February 2024
View articletitled, Excitation energy transfer in proteoliposomes reconstituted with LH2 and RC-LH1 complexes from Rhodobacter sphaeroides
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Includes: Supplementary data
Articles

Commentary on: Divalent metal cofactors differentially modulate RadA-mediated strand invasion and exchange in Saccharolobus solfataricus

Irene C. Mangialavori
Journal: Bioscience Reports
Biosci Rep (2023) 43 (6): BSR20230058.
https://doi.org/10.1042/BSR20230058
Published: 28 June 2023
View articletitled, Commentary on: Divalent metal cofactors differentially modulate RadA-mediated strand invasion and exchange in Saccharolobus solfataricus
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Articles

Expression and characterisation of human glycerol kinase: the role of solubilising agents and molecular chaperones

Riva Mary Rani, Superior Syngkli, Joplin Nongkhlaw, Bidyadhar Das
Journal: Bioscience Reports
Biosci Rep (2023) 43 (4): BSR20222258.
https://doi.org/10.1042/BSR20222258
Published: 21 April 2023
View articletitled, Expression and characterisation of human glycerol kinase: the role of solubilising agents and molecular chaperones
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Includes: Supplementary data
Articles

Engineering of enzymes using non-natural amino acids

In Collection
Bionanotechnology and Synthetic Biology: applications in precision medicine and allied fields
Yiwen Li, Paul A. Dalby
Journal: Bioscience Reports
Biosci Rep (2022) 42 (8): BSR20220168.
https://doi.org/10.1042/BSR20220168
Published: 05 August 2022
View articletitled, Engineering of enzymes using non-natural amino acids
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Articles

Comparing the folding landscapes of evolutionarily divergent procaspase-3

Liqi Yao, A. Clay Clark
Journal: Bioscience Reports
Biosci Rep (2022) 42 (6): BSR20220119.
https://doi.org/10.1042/BSR20220119
Published: 17 June 2022
View articletitled, Comparing the folding landscapes of evolutionarily divergent procaspase-3
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Includes: Supplementary data
Articles

mem-iLID, a fast and economic protein purification method

Ruijing Tang, Shang Yang, Georg Nagel, Shiqiang Gao
Journal: Bioscience Reports
Biosci Rep (2021) 41 (7): BSR20210800.
https://doi.org/10.1042/BSR20210800
Published: 28 June 2021
View articletitled, mem-iLID, a fast and economic protein purification method
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Includes: Supplementary data
Articles

Evaluation of spice and herb as phyto-derived selective modulators of human retinaldehyde dehydrogenases using a simple in vitro method

Thi Bao Chau Bui, Shohei Nosaki, Mito Kokawa, Yuqun Xu, Yutaka Kitamura, Masaru Tanokura, Satoshi Hachimura, Takuya Miyakawa
Journal: Bioscience Reports
Biosci Rep (2021) 41 (5): BSR20210491.
https://doi.org/10.1042/BSR20210491
Published: 20 May 2021
View articletitled, Evaluation of spice and herb as phyto-derived selective modulators of human retinaldehyde dehydrogenases using a simple in vitro method
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Includes: Supplementary data
Articles

Pyruvate dehydrogenase kinases (PDKs): an overview toward clinical applications

Xiuxiu Wang, Xiaoyue Shen, Yuting Yan, Hongmin Li
Journal: Bioscience Reports
Biosci Rep (2021) 41 (4): BSR20204402.
https://doi.org/10.1042/BSR20204402
Published: 07 April 2021
View articletitled, Pyruvate dehydrogenase kinases (PDKs): an overview toward clinical applications
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Articles

Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N 6 -methyladenine backgrounds in Escherichia coli

Zheng Chen, Yan Liu, Hailin Wang
Journal: Bioscience Reports
Biosci Rep (2021) 41 (3): BSR20203769.
https://doi.org/10.1042/BSR20203769
Published: 15 March 2021
View articletitled, Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N 6 -methyladenine backgrounds in Escherichia coli
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Includes: Supplementary data
Articles

Remodeling hydrogen bond interactions results in relaxed specificity of Caspase-3

In Collection
Protein structure and function
Liqi Yao, Paul Swartz, Paul T. Hamilton, A. Clay Clark
Journal: Bioscience Reports
Biosci Rep (2021) 41 (1): BSR20203495.
https://doi.org/10.1042/BSR20203495
Published: 29 January 2021
View articletitled, Remodeling hydrogen bond interactions results in relaxed specificity of Caspase-3
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Includes: Supplementary data
Articles

Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9

Chun Hang Lau, Edward L. Bolt
Journal: Bioscience Reports
Biosci Rep (2021) 41 (1): BSR20203595.
https://doi.org/10.1042/BSR20203595
Published: 05 January 2021
View articletitled, Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9
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Includes: Supplementary data
Articles

Promiscuous DNA cleavage by HpyAII endonuclease is modulated by the HNH catalytic residues

Sumith Kumar, Sushant Bangru, Ritesh Kumar, Desirazu N. Rao
Journal: Bioscience Reports
Biosci Rep (2020) 40 (9): BSR20201633.
https://doi.org/10.1042/BSR20201633
Published: 16 September 2020
View articletitled, Promiscuous DNA cleavage by HpyAII endonuclease is modulated by the HNH catalytic residues
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Includes: Supplementary data
Articles

Occupation of a thermoresistant-scaffold (αRep) at SP1-NC cleavage site disturbs the function of HIV-1 protease

Sudarat Hadpech, Nichakan Peerakam, Koollawat Chupradit, Chatchai Tayapiwatana
Journal: Bioscience Reports
Biosci Rep (2020) 40 (6): BSR20201131.
https://doi.org/10.1042/BSR20201131
Published: 23 June 2020
View articletitled, Occupation of a thermoresistant-scaffold (αRep) at SP1-NC cleavage site disturbs the function of HIV-1 protease
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Articles

Quantitative iTRAQ LC-MS/MS reveals muscular proteome profiles of deep pressure ulcers

Zan Liu, Xu Cui, Yanke Hu, Pihong Zhang
Journal: Bioscience Reports
Biosci Rep (2020) 40 (6): BSR20200563.
https://doi.org/10.1042/BSR20200563
Published: 15 June 2020
View articletitled, Quantitative iTRAQ LC-MS/MS reveals muscular proteome profiles of deep pressure ulcers
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Includes: Supplementary data
Articles

Comparative study on hemoglobin A1c, glycated albumin and glycosylated serum protein in aplastic anemia patients with Type 2 diabetes mellitus

Minghuan Suo, Dongmei Wen, Weijia Wang, Tingting Zhang
Journal: Bioscience Reports
Biosci Rep (2020) 40 (5): BSR20192300.
https://doi.org/10.1042/BSR20192300
Published: 22 May 2020
View articletitled, Comparative study on hemoglobin A1c, glycated albumin and glycosylated serum protein in aplastic anemia patients with Type 2 diabetes mellitus
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Articles

Superior adjuvanticity of the genetically fused D1 domain of Neisseria meningitides Ag473 lipoprotein among three Toll-like receptor ligands

Huipeng Lu, Xiaokai Zhang, Yuyang Wang, Yang Zong, Yajie Wang, Xinyu Zhang, Xiaoli Xia, Huaichang Sun
Journal: Bioscience Reports
Biosci Rep (2020) 40 (4): BSR20193675.
https://doi.org/10.1042/BSR20193675
Published: 07 April 2020
View articletitled, Superior adjuvanticity of the genetically fused D1 domain of Neisseria meningitides Ag473 lipoprotein among three Toll-like receptor ligands
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Includes: Supplementary data
Articles

Trimeric heptad repeat synthetic peptides HR1 and HR2 efficiently inhibit HIV-1 entry

Olfa Mzoughi, Meritxell Teixido, Rémi Planès, Manutea Serrero, Ibtissem Hamimed, Esther Zurita, Miguel Moreno, Giovana Granados, Faouzi Lakhdar-Ghazal, Lbachir BenMohamed, Ernest Giralt, Elmostafa Bahraoui
Journal: Bioscience Reports
Biosci Rep (2019) 39 (9): BSR20192196.
https://doi.org/10.1042/BSR20192196
Published: 24 September 2019
View articletitled, Trimeric heptad repeat synthetic peptides HR1 and HR2 efficiently inhibit HIV-1 entry
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Articles

Changing substrate specificity and iteration of amino acid chain elongation in glucosinolate biosynthesis through targeted mutagenesis of Arabidopsis methylthioalkylmalate synthase 1

Annette Petersen, Lea Gram Hansen, Nadia Mirza, Christoph Crocoll, Osman Mirza, Barbara Ann Halkier
Journal: Bioscience Reports
Biosci Rep (2019) 39 (7): BSR20190446.
https://doi.org/10.1042/BSR20190446
Published: 02 July 2019
View articletitled, Changing substrate specificity and iteration of amino acid chain elongation in glucosinolate biosynthesis through targeted mutagenesis of Arabidopsis methylthioalkylmalate synthase 1
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Includes: Supplementary data
Articles

Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland

Rui Lu, Ting Zhang, Shaozheng Song, Minya Zhou, Lei Jiang, Zhengyi He, Yuguo Yuan, Tingting Yuan, Yaoyao Lu, Kunning Yan, Yong Cheng
Journal: Bioscience Reports
Biosci Rep (2019) 39 (6): BSR20190596.
https://doi.org/10.1042/BSR20190596
Published: 28 June 2019
View articletitled, Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland
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Articles

Fructose 1,6- bis phosphatase: getting the message across

David J. Timson
Journal: Bioscience Reports
Biosci Rep (2019) 39 (3): BSR20190124.
https://doi.org/10.1042/BSR20190124
Published: 06 March 2019
View articletitled, Fructose 1,6- bis phosphatase: getting the message across
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Articles

Engineering enzyme catalysis: an inverse approach

Clare F. Megarity
Journal: Bioscience Reports
Biosci Rep (2019) 39 (2): BSR20181107.
https://doi.org/10.1042/BSR20181107
Published: 12 February 2019
View articletitled, Engineering enzyme catalysis: an inverse approach
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Articles

Functional and structural studies of tolloid-like 1 mutants associated with atrial-septal defect 6

Lukasz Sieron, Marta Lesiak, Izabela Schisler, Zofia Drzazga, Andrzej Fertala, Aleksander L. Sieron
Journal: Bioscience Reports
Biosci Rep (2019) 39 (1): BSR20180270.
https://doi.org/10.1042/BSR20180270
Published: 08 January 2019
View articletitled, Functional and structural studies of tolloid-like 1 mutants associated with atrial-septal defect 6
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Includes: Supplementary data
Articles

NAD(P)H quinone oxidoreductase (NQO1): an enzyme which needs just enough mobility, in just the right places

Angel L. Pey, Clare F. Megarity, David J. Timson
Journal: Bioscience Reports
Biosci Rep (2019) 39 (1): BSR20180459.
https://doi.org/10.1042/BSR20180459
Published: 03 January 2019
View articletitled, NAD(P)H quinone oxidoreductase (NQO1): an enzyme which needs just enough mobility, in just the right places
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Articles

An efficient method for FITC labelling of proteins using tandem affinity purification

Lalith K. Chaganti, Navneet Venkatakrishnan, Kakoli Bose
Journal: Bioscience Reports
Biosci Rep (2018) 38 (6): BSR20181764.
https://doi.org/10.1042/BSR20181764
Published: 11 December 2018
View articletitled, An efficient method for FITC labelling of proteins using tandem affinity purification
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Articles

Role of Annexin A2 isoform 2 on the aggregative growth of dermal papillae cells

Jing Gu, Yinni Ma, Lijia Yang, Feng Wang, Cao Lei, Jianxin Zhai, Rushan Xia
Journal: Bioscience Reports
Biosci Rep (2018) 38 (6): BSR20180971.
https://doi.org/10.1042/BSR20180971
Published: 07 December 2018
View articletitled, Role of Annexin A2 isoform 2 on the aggregative growth of dermal papillae cells
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Includes: Supplementary data
Articles

LncRNA PROX1-AS1 promotes proliferation, invasion, and migration in papillary thyroid carcinoma

Yanyan Shen, Erjie Xia, Adheesh Bhandari, Xiaohui Wang, Guilong Guo
Journal: Bioscience Reports
Biosci Rep (2018) 38 (5): BSR20180862.
https://doi.org/10.1042/BSR20180862
Published: 12 September 2018
View articletitled, LncRNA PROX1-AS1 promotes proliferation, invasion, and migration in papillary thyroid carcinoma
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Articles

Apolipoprotein E deletion has no effect on copper-induced oxidative stress in the mice brain

Yuan Chen, Liang Wang, Jiang-hui Geng, Hui-feng Zhang, Li Guo
Journal: Bioscience Reports
Biosci Rep (2018) 38 (5): BSR20180719.
https://doi.org/10.1042/BSR20180719
Published: 07 September 2018
View articletitled, Apolipoprotein E deletion has no effect on copper-induced oxidative stress in the mice brain
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Articles

Chitinase: diversity, limitations, and trends in engineering for suitable applications

Ayokunmi Oyeleye, Yahaya M. Normi
Journal: Bioscience Reports
Biosci Rep (2018) 38 (4): BSR2018032300.
https://doi.org/10.1042/BSR20180323
Published: 29 August 2018
View articletitled, Chitinase: diversity, limitations, and trends in engineering for suitable applications
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Articles

Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers

Michelle Simons, Svetlana Gretton, Gary G.A. Silkstone, Badri S. Rajagopal, Victoria Allen-Baume, Natalie Syrett, Thoufieq Shaik, Nelida Leiva-Eriksson, Luca Ronda, Andrea Mozzarelli, Michael B. Strader, Abdu I. Alayash, Brandon J. Reeder, Chris E. Cooper
Journal: Bioscience Reports
Biosci Rep (2018) 38 (4): BSR20180370.
https://doi.org/10.1042/BSR20180370
Published: 03 July 2018
View articletitled, Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers
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Includes: Supplementary data
Articles

Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

Johanna Hausjell, Heidi Halbwirth, Oliver Spadiut
Journal: Bioscience Reports
Biosci Rep (2018) 38 (2): BSR20171290.
https://doi.org/10.1042/BSR20171290
Published: 05 March 2018
View articletitled, Recombinant production of eukaryotic cytochrome P450s in microbial cell factories
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Articles

The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation

Mir Hussain Nawaz, Juliana C. Ferreira, Lyudmila Nedyalkova, Haizhong Zhu, César Carrasco-López, Serdal Kirmizialtin, Wael M. Rabeh
Journal: Bioscience Reports
Biosci Rep (2018) 38 (1): BSR20171666.
https://doi.org/10.1042/BSR20171666
Published: 21 February 2018
View articletitled, The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation
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Includes: Supplementary data
Articles

An amperometric H 2 O 2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode

Vinay Narwal, Neelam Yadav, Manisha Thakur, Chandra S. Pundir
Journal: Bioscience Reports
Biosci Rep (2017) 37 (4): BSR20170194.
https://doi.org/10.1042/BSR20170194
Published: 17 July 2017
View articletitled, An amperometric H 2 O 2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode
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Articles

Effects of microvirin monomers and oligomers on hepatitis C virus

Yuan-Qin Min, Xu-Chu Duan, Yi-Dan Zhou, Anna Kulinich, Wang Meng, Zhi-Peng Cai, Hong-Yu Ma, Li Liu, Xiao-Lian Zhang, Josef Voglmeir
Journal: Bioscience Reports
Biosci Rep (2017) 37 (3): BSR20170015.
https://doi.org/10.1042/BSR20170015
Published: 30 June 2017
View articletitled, Effects of microvirin monomers and oligomers on hepatitis C virus
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Includes: Supplementary data
Articles

The integrin-binding defective FGF2 mutants potently suppress FGF2 signalling and angiogenesis

Seiji Mori, Nobuaki Hatori, Naomasa Kawaguchi, Yoshinosuke Hamada, Tsung-Chieh Shih, Chun-Yi Wu, Kit S. Lam, Nariaki Matsuura, Hirofumi Yamamoto, Yoko K. Takada, Yoshikazu Takada
Journal: Bioscience Reports
Biosci Rep (2017) 37 (2): BSR20170173.
https://doi.org/10.1042/BSR20170173
Published: 10 April 2017
View articletitled, The integrin-binding defective FGF2 mutants potently suppress FGF2 signalling and angiogenesis
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Articles

Computational approaches for de novo design and redesign of metal-binding sites on proteins

Gunseli Bayram Akcapinar, Osman Ugur Sezerman
Journal: Bioscience Reports
Biosci Rep (2017) 37 (2): BSR20160179.
https://doi.org/10.1042/BSR20160179
Published: 27 March 2017
View articletitled, Computational approaches for de novo design and redesign of metal-binding sites on proteins
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Articles

Protein kinase C β inhibits autophagy and sensitizes cervical cancer Hela cells to cisplatin

Na Li, Wei Zhang
Journal: Bioscience Reports
Biosci Rep (2017) 37 (2): BSR20160445.
https://doi.org/10.1042/BSR20160445
Published: 27 March 2017
View articletitled, Protein kinase C β inhibits autophagy and sensitizes cervical cancer Hela cells to cisplatin
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Articles

Different specificities of two aldehyde dehydrogenases from Saccharomyces cerevisiae var. boulardii

Suprama Datta, Uday S. Annapure, David J. Timson
Journal: Bioscience Reports
Biosci Rep (2017) 37 (2): BSR20160529.
https://doi.org/10.1042/BSR20160529
Published: 02 March 2017
View articletitled, Different specificities of two aldehyde dehydrogenases from Saccharomyces cerevisiae var. boulardii
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Includes: Supplementary data
Articles

Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA

Benjamin W. Arentson, Erin L. Hayes, Weidong Zhu, Harkewal Singh, John J. Tanner, Donald F. Becker
Journal: Bioscience Reports
Biosci Rep (2016) 36 (6): e00413.
https://doi.org/10.1042/BSR20160435
Published: 22 November 2016
View articletitled, Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA
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