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Keywords: CRISPR/Cas9
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Articles
Journal:
Bioscience Reports
Biosci Rep (2019) 39 (10): BSR20191914.
Published: 15 October 2019
...Hong Pan; Weina Yu; Ming Zhang Precise genome editing with directed base insertion or targeted point mutations can be achieved by CRISPR/Cas9-mediated homology-directed repair (HDR) and is of great significance in clinical disease therapy. However, HDR efficiency, compared with non-homologous end...
Includes: Supplementary data
Articles
Ting Zhang, Yaoyao Lu, Shaozheng Song, Rui Lu, Minya Zhou, Zhengyi He, Tingting Yuan, Kunning Yan, Yong Cheng
Journal:
Bioscience Reports
Biosci Rep (2019) 39 (5): BSR20190207.
Published: 21 May 2019
... ) gene can cause the ‘double-muscling’ phenotype. Although there have been many studies performed on MSTN -mutant animals, there have been few studies that have investigated the cystine-knot motif in exon 3 of MSTN in rabbits. In the current study, CRISPR/Cas9 sgRNA anchored exon 3 of a rabbit’s MSTN...
Includes: Supplementary data
Articles
Journal:
Bioscience Reports
Biosci Rep (2018) 38 (6): BSR20180742.
Published: 14 November 2018
...-muscled (DM) phenotype. MSTN mutation animal, such as sheep, dog, and rabbit have been generated through CRISPR/Cas9 technology. However, goats with promising MSTN mutation have not been generated. We designed two sgRNAs loci targetting exon3 of MSTN gene to destroy the MSTN cysteines knots. We got seven...
Includes: Supplementary data
Articles
Journal:
Bioscience Reports
Biosci Rep (2018) 38 (2): BSR20171490.
Published: 21 March 2018
..., gonad development, or expression of hormones and their receptors during embryonic development during sexual differentiation. SRY is a sex-determination gene on the Y chromosome that is responsible for initiating male sex determination in mammals. In this study, we introduced CRISPR/Cas9-mediated...
Includes: Supplementary data