... to develop three stages of goose GCs in vitro mono-culture and co-culture models, and we analyzed the morphology, activity, intracellular lipid content and the expression of core genes involved in de novo lipogenesis (DNL), steroidogenesis, proliferation and apoptosis of the GCs. In the co-culture group...

... measurement. Equal amount of total RNA (1 μg) from each sample was reversely transcribed into cDNA using a cDNA synthesis kit following the manufacture’s instruction (TaKaRa, Shiga, Japan). Follicle development Goose Granulosa cell Lipid metabolism As the critical component of ovarian...