... sensitive Mat α; leu2::hisG; trp1::hisG, his3::hisG; ura352 aqy1D::KanMX aqy2D::KanMX aquaporins drug discovery and design high-throughput screening ion channels Saccharomyces cerevisiae Australian Research Council 19ARC_DP190101745 Following the first discovery...

... using vacuoles are being actively conducted. The practical application of the present study to animals requires the determination of the biocompatibility of vacuole. In the present study, we evaluated the effects of vacuoles isolated from Saccharomyces cerevisiae in RAW 264.7 cells. This showed...

... Version of Record. You are encouraged to use the final Version of Record that, when published, will replace this manuscript and be freely available under a Creative Commons licence. Saccharomyces cerevisiae Vacuole RAW264.7 Cytokine Immunostimulatory activity 1 2 3 Enhanced Immune Response...

... and distributed under the Creative Commons Attribution License 4.0 (CC BY) . acetyltransferase MAK3 N-terminal acetylation NAA30 Saccharomyces cerevisiae stress response Given the prevalence and impact of N-terminal acetylation, it will be important to assess the pathogenic nature of newly...

...Callum J. O’Kane; Edel M. Hyland Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) are two of the most recognised and well-studied model systems for epigenetic regulation and the inheritance of chromatin states. Their silent loci serve as a proxy...

... production. This review describes current strategies for expression of P450s in the microbial cell factories Escherichia coli, Saccharomyces cerevisiae , and Pichia pastoris . Most eukaryotic P450s are membrane bound, the majority of them being natively located in the endoplasmic reticulum, whereas...

.... Here we present the crystal structure of an N-terminal fragment of Saccharomyces cerevisiae Hsp104 exhibiting molecular interactions involving both AAA-1 pore loops, which resemble contacts with bound substrate. Corroborated by biochemical experiments and functional studies in yeast, we show...

... for GPI-T. Saccharomyces cerevisiae INV (invertase), a soluble secreted protein, was converted into a substrate for GPI-T by appending the C-terminal 21 amino acid GPI-T signal sequence from the S. cerevisiae Yapsin 2 [Mkc7p (Y21)] on to the C-terminus of INV. Using a colorimetric assay and biochemical...

...Lidiya Lichko; Tatyana Kulakovskaya; Nikolai Pestov; Igor Kulaev Purified fractions of cytosol, vacuoles, nuclei, and mitochondria of Saccharomyces cerevisiae possessed inorganic polyphosphates with chain lengths characteristic of each individual compartment. The most part (80–90%) of the total...

...William Mann; Jonathan Jeffery Efficient preparation of spheroplasts from Candida utilis, Saccharomyces cerevisiae , and Schizosaccharomyces pombe , using a purified mixture of enzymes from Trichoderma harzianum , is described. Limitations of other methods, and differences between yeasts...