... dehydrogenase from Pto DC3000. Site-directed mutants targeting the catalytic residues Cys 302 and Glu 267 resulted in a loss of enzymatic activity. The X-ray crystal structure of the catalytically inactive AldA C302A mutant in complex with IAA and NAD + showed the cofactor adopting a conformation that differs...

... . In this work, the high-resolution crystal structures of IspF in native and complex with CMP from B. subtilis have been determined. Structural comparisons indicate that there is a looser packing of the subunits of IspF in B. subtilis , whereas the solvent accessible surface of its active pockets is smaller than...

..., it has a potential application of SDS biodegradation. The crystal structure of SdsAP has been solved to a resolution of 1.76 Å and reveals that SdsAP contains the characteristic metallo-β-lactamase-like fold domain, dimerization domain, and C-terminal sterol carrier protein type 2 (SCP-2)-like fold...

... and equine serum albumins based on two crystal structures are presented. During the last two decades it has been shown that lysophospholipids (LPLs) are important signalling molecules involved in many physiological and pathophysiological processes [ 1 ]. So far, the best characterized LPLs...

.... The 1.50 Å crystal structure of Mm NQO features a homodimeric enzyme where each monomer comprises 196 residues folding into flavodoxin-like α/β domains with non-covalently bound FMN (flavin mononucleotide). The closest structural homologue is the modulator of drug activity B from Streptococcus mutans...

... that several of the residues in the G5 motif have an important role in the intrinsic affinity and release of GDP. In particular, a T151A mutant (third residue of the G5 loop) leads to a reduced nucleotide affinity and provokes a drastically accelerated dissociation of GDP. crystal structure GDP release...

... repetitive RII (Region II). Mp AFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously determined that a single RII repeat folds as a Ca 2+ -dependent immunoglobulin-like domain. Here, we solved the crystal structure of RII tetra-tandemer (four tandem RII repeats...

... the protein and the mechanism may provide insights into destabilization and the formation of aggregated proteins. Here, we determined the crystal structure of 2-ME-SOD1 and find that the 2-ME moieties in both subunits interact asymmetrically at the dimer interface and that there is an asymmetric configuration...

.... The relevant conformations of this domain during the different steps of the catalytic cycle are still under discussion. Through crystal structures of the apo- and phosphate-bound ATPBDs, with limited proteolysis and fluorescence studies of the apo- and substrate-bound states, we show that the isolated ATPBD...

... in enzyme activity. The tryptophan fluorescence change at excitation/emission=280/350 nm upon L2–L2′ loop cleavage was found to be higher in catalytically active mutants, including the corresponding wild-type caspase, than in the inactive mutants. The crystal structures of the caspase 3 mutants were solved...

... aminotransferase crystal structure oxo acid kynurenic acid kynurenine kynurenine aminotransferase KYNA (kynurenic acid) is the only known endogenous antagonist of the NMDA ( N -methyl- D -aspartate) subtype of glutamate receptors [ 1 – 4 ]. KYNA is also the antagonist of the α7-nicotinic acetylcholine...

... 2008 Biochemical Society 2008 aminoadipic acid crystal structure kynurenic acid (KYNA) kynurenine kynurenine aminotransferase (KAT) neurodegenerative disease 1 To whom correspondence should be addressed (email [email protected] ). Aminotransferase, capable of catalysing...