... affect neuronal development independently of its effects on the vasculature. To test this hypothesis, the current study differentiated the human neural progenitor cell (NPC) line ReNcell® VM into a mixed culture of mature neurons and glia, and exposed them to sFlt-1 during development. Outcomes measured...

... © 2021 The Author(s). 2021 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . adipogenesis chicken differentiation fatty acid inducer preadipocyte...

..., proliferation, migration and cell differentiation culminates in cell elongation and organelle degradation, allowing the light to reach the retina. HOPS ( H epatocyte O dd P rotein S huttling)/TMUB1 ( T rans M embrane Ub iquitin-like containing protein 1 ) is a nucleo-cytoplasmic shuttling protein, highly...

... to analyze the expression of related miRNA in the aortic dissection of AD patients and healthy controls. Identification of differentially expressed (DE)-miRNA was performed by Limmar package (Fold Change = −2.16, P =0.01597). starBase v2.0 software was used to predict the potential targeted binding sites...

... of migration, proliferation and differentiation. MicroRNAs (miRNAs) are small non-coding RNAs that play important roles on regulating various cell behaviors. Here, we report that miRNA-124 (miR124) and miRNA-21-5p (miR21-5p) display different regulatory roles on migration, proliferation and neuron...

...-β1 treatment dose-dependently curbed miR-29b-3p expression in CFs. miR-29b-3p restrained the promotive impacts of TGF-β1 on CFs proliferation, migration, and differentiation. FOS was affirmed to be a target of miR-29b-3p, elevated expression of FOS reversed the inhibitory effects of miR-29b-3p...

...Suxuan Liu; Wen Ke; Yang Liu; Zhenzhen Zhao; Lina An; Xiaohua You; Fan Yang; Xiangqun Yang; Guokun Wang; Xianxian Zhao Background: Cardiac fibroblasts differentiation plays a critical role in cardiac remodeling and failure, but the underlying molecular mechanisms are still poorly understood...

....; no. SA00008-9; [PE] labeled; dilution ratio, 1:100). chicken differentiation Histone acetylation Spermatogonial stem cell Sox2 Spermatogonial stem cell (SSCs) is the only adult stem cell in the body that can transmit genetic information to their offspring. They have the potential...

...Anita Ferraretto; Michela Bottani; Paola De Luca; Laura Cornaghi; Francesca Arnaboldi; Margherita Maggioni; Amelia Fiorilli; Elena Donetti An intestinal 70/30 Caco2/HT-29 co-culture was set up starting from the parental populations of differentiated cells to mimic the human intestinal epithelium...

... extracts. The microspheres had a smooth surface and were spherical in shape. The average diameter of the microspheres was 3.95 µm. The loading efficiency was approximately 2.12%. The purity of isolated hBMSC was over 98.8%. Rg1-loaded microspheres could promote hBMSC proliferation and differentiation...

...). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA...

...Bohan Cheng; Mengqi Wu; Songsong Xu; Xinyang Zhang; Yuxiang Wang; Ning Wang; Li Leng; Hui Li The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation...

... cysteines. Previously, we reported that the classical form of cytosolic TrxR1 (TXNRD1_v1), when overexpressed in human embryonic kidney cells (HEK-293), prompted the cells to undergo differentiation [Nalvarte et al. (2004) J. Biol. Chem. 279 , 54510–54517]. In the present study, we show that several genes...

... study, miR-146 overexpression and predicted target (notch 1) were used to study proliferation and differentiation of mouse NSCs. shRNA were used to demonstrate the function of Notch 1 in proliferation of mouse NSCs and luciferase reporter assay was used to assess and confirm the binding sequence of 3...

... neuroblast divides asymmetrically to produce a larger daughter cell that retains neuroblast identity, and a smaller daughter cell that is committed to undergo differentiation. Neuroblast self-renewal and differentiation are tightly controlled by a set of intrinsic factors that regulate ACD (asymmetric cell...

...Zhouchun Shang; Lin Guo; Ning Wang; Hui Shi; Yuxiang Wang; Hui Li In addition to providing energy and constituting cell membrane, fatty acids also play an important role in adipocyte differentiation and lipid metabolism. As an important member of monounsaturated fatty acids, oleate, together...

... observed the gene expression of pluripotent markers in foetal pMSCs and the capacity of pMSCs to differentiate into adipocytes, osteocytes and neural-like cells using quantitative RT–PCR (reverse transcription–PCR), normal histological staining and immunohistochemistry. Foetal pMSCs have either a spindle...

... is spatially and temporally regulated to act on Ras family GTPases Rap1, Rap2, R-Ras, TC21 and Rho family member TC10. Increased C3G protein levels are associated with differentiation of various cell types, indicating an important role for C3G in cellular differentiation. In signalling pathways, C3G serves...

... and differentiation. Moreover, perturbation of those chromatin regulators results in loss of ES cell identity or aberrant differentiation. Therefore, it is important to fully understand the chromatin regulation landscape of ES cells. The knowledge gained will help us to harness the unique characteristics of ES cells...

...-differentiation of stem cells on the PNIPAAm-grafted PDMS surface. The results showed that hMSCs could adhere on the PNIPAAm-grafted PDMS surface at 37°C and form cell colonies, and then become fibroblastic. The proliferation potential of hMSCs on the PNIPAAm-grafted PDMS surface was not significantly different...

... disorders are also reported. © 2007 The Biochemical Society 2007 Mitochondria Stem cells Differentiation Mitochondrial localization ROS MNGIE MINI REVIEW The Role of Mitochondria in Stem Cell Biology Claudia Nesti Æ Livia Pasquali Æ Francesca Vaglini Æ Gabriele Siciliano Æ Luigi Murri...

... of the liquid matrix of cell membranes appears to be responsible for its effects and this appears also to be true for related effects on membrane permeability and fusion. Dimethyl sulphoxide is also known to act as an inducer of cellular differentiation and as a free radical scavenger and radioprotectant...

...Ewa Rydell; Karl-Eric Magnusson; Anita Sjö; Krister Axelsson Protein kinase C (PK-C) and casein kinase II (CK-II) activities were studied in two human colon carcinoma cell lines (HT-29 and CaCO-2) undergoing differentiation in vitro resulting, in small-intestine-like cells. CaCo-2 cells, when grown...

...Elvira Cuenllas; Soledad Gaitan; Juan A. Bueren; Concepcion Tejero Pyruvate kinase studies in the granulocyte-macrophage lineage during in vitro differentiation have been performed using culture techniques on GM-CFC cells and a study has also been done in bone marrow cells. The enzyme exhibits...

...Takashi Momoi; Haruki Senoo; Hiroshi Yoshikura The differentiation of HL-60 cells induced by 1α,25 dihydroxyvitamin D 3 was found to be separated into two stages, i.e. commitment and promotion. Most of the HL-60 cells were committed to monocyte/macrophage lineage by pretreatment with 1α,25...