... dissolved air. Enzyme concentration of 10 μM was used for UCHL1 C90S and UCHL5 C95S and ubiquitin concentration used for both was 100 μM. For UCHL5 C88S and BAP1N C91S concentrations used were 250 and 120 μM, respectively, ubiquitin used in the present study was ten-fold higher concentration than...

... a cell wall composed of chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A. fumigatus possesses an active Af AGM1 ( A. fumigatus N -acetylphosphoglucosamine mutase), a key enzyme...

... anhydride in APS (adenosine 5′-phosphosulfate) from ATP and sulfate as the first committed step of sulfur assimilation in plants. In contrast to the multi-functional, allosterically regulated ATP sulfurylases from bacteria, fungi and mammals, the plant enzyme functions as a mono-functional, non-allosteric...

...Hongpeng He; Mei-Chin Lee; Li-Ling Zheng; Lei Zheng; Yan Luo The concept of one-protein–multiple-function, i.e. moonlighting proteins, is an ever-expanding paradigm. We obtained compelling evidence that an array of ‘cytoplasmic’ metabolic enzymes can enter the nuclei to carry out moonlighting...

... kinetic parameters that can explain data on the enzyme-catalysed reaction kinetics have not been established. To determine an accurate model, a set of putative mechanisms of SCS, proposed by previous researchers, were tested against experimental data (from previous publication) on SCS derived from porcine...