... approach for the investigation of biochemical pathways. Model simulations can guide experiments and support the identification of potential drug targets. Their construction relies on the availability of experimental data concerning the detailed enzymatic mechanisms, the enzyme kinetics, the associated...

... of incubation. FXIIa activity was calculated using the slope of the linear regression curve, and converted to initial velocity (in nM of PKa/s/nM of FXIIa). bradykinin enzyme kinetics kallikreins serine proteases In the MM model, the two steps in ( eqn E1 ) were iteratively simulated...

... The Author(s). 2022 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . cancer enzyme activity enzyme kinetics enzymology molecular mechanisms Over 100 years...

... Commons Attribution License 4.0 (CC BY) . allosteric regulation enzyme kinetics lactate dehydrogenase lactic acid liver Lactic acid is an important component of the energetic metabolism, in both prokaryotic and eukaryotic organisms. Among prokaryotes, lactic acid bacteria share...

... 2020 26 03 2020 02 04 2020 © 2020 The Author(s). 2020 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Enzyme kinetics estradiol glycolysis...

... and distributed under the Creative Commons Attribution License 4.0 (CC BY) . enzyme kinetics enzyme-substrate interactions enzyme activity insulin metalloproteases protease inhibitor Scientific progress goes hand in hand with analytical advances. Nowadays, in many fields of science, new...

... analysed utilizing ProteinPilot™ software ( http://www.absciex.com/products/software/proteinpilot-software ) for peptide sequence identification. In some experiments, LC MS was carried out with purified unphosphorylated/phosphorylated histidine-tagged proteins for determining intact mass. enzyme...

... 13 10 2016 enzyme kinetics Methanobrevibacter smithii NADH oxidase protein function Recently, a role of the gut microbiota has been attracting more attention. Besides the many bacterial species, the human gut contains several archaeal species but only three distinct species...

... is also reported. antibiotic sensitivity enzyme kinetics Francisella tularensis kinase polyphosphate X-ray crystallography 3 8 2015 28 9 2015 28 10 2015 18 11 2015 © 2016 Authors 2016 This is an open access article published by Portland Press Limited...

... of adenosylcobalamin. Using a range of biophysical techniques, we have obtained molecular details on substrate binding and investigated protein stability. Kinetic experiments have identified half-site reactivity. enzyme kinetics enzyme structure NMR vitamins and cofactors X-ray crystallography...