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Keywords: exocytosis
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Articles
Micaela Vanina Buzzatto, Fabiana Cristina Benegas Guerrero, Pablo Ariel Álvarez, María Paz Zizzias, Luis Mariano Polo, Claudia Nora Tomes
Journal:
Bioscience Reports
Biosci Rep (2024) 44 (7): BSR20240117.
Published: 30 July 2024
...Micaela Vanina Buzzatto; Fabiana Cristina Benegas Guerrero; Pablo Ariel Álvarez; María Paz Zizzias; Luis Mariano Polo; Claudia Nora Tomes Botulinum neurotoxins (BoNTs) are valuable tools to unveil molecular mechanisms of exocytosis in neuronal and non-neuronal cells due to their peptidase activity...
Includes: Supplementary data
Articles
Luciano G. Dolce, Rui M. P. Silva-Junior, Leandro H. P. Assis, Andrey F. Z. Nascimento, Jackeline S. Araujo, Ingrid P. Meschede, Enilza M. Espreafico, Priscila O. de Giuseppe, Mário T. Murakami
Journal:
Bioscience Reports
Biosci Rep (2019) 39 (3): BSR20182189.
Published: 01 March 2019
... the purification in a HiLoad Superdex 200 16/60 column (GE Healthcare) pre-equilibrated with SEC buffer. cellular localization exocytosis myosins protein-protein interactions trafficking transcription Class V myosins are processive motors that transport or tether vesicles, organelles...
Includes: Supplementary data
Articles
Binhui Ren, Zoulikha Azzegagh, Ana M. Jaramillo, Yunxiang Zhu, Ana Pardo-Saganta, Rustam Bagirzadeh, Jose R. Flores, Wei Han, Yong-jun Tang, Jing Tu, Denise M. Alanis, Christopher M. Evans, Michele Guindani, Paul A. Roche, Jayaraj Rajagopal, Jichao Chen, C. William Davis, Michael J. Tuvim, Burton F. Dickey
Journal:
Bioscience Reports
Biosci Rep (2015) 35 (3): e00220.
Published: 25 June 2015
.... Dickey Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. We find the trafficking protein, SNAP23 (23-kDa paralogue of synaptosome-associated protein of 25 kDa), selectively expressed in secretory cells compared with ciliated and basal...
Includes: Supplementary data
Articles
Journal:
Bioscience Reports
Biosci Rep (2015) 35 (3): e00203.
Published: 11 June 2015
... labelled fluorescence probes) is useful for detecting the DNA fragments generated by DNase II in Caenorhabditis elegans embryos. It reveals ~70% ToLFP signals in dying cells and 30% in engulfing cells during embryogenesis. apoptosis Caenorhabditis elegans DNase II exocytosis fluorescence...
Includes: Multimedia, Supplementary data
Articles
A Study of Membrane Activity in Rat Prostate Cancer Cells: An Evaluation of the FM1–43 Dye Technique
Journal:
Bioscience Reports
Biosci Rep (2000) 20 (1): 31–40.
Published: 01 February 2000
... FM1–43 membranes cancer prostate Danning model endocytosis exocytosis Bioscience Reports, Vol. 20, No. 1, 2000 A Study of Membrane Activity in Rat Prostate Cancer Cells: An Evaluation of the FM1-43 Dye Technique Maria E. Mycielska,1,2 Monika Scha¨fer,3 Scott P. Fraser,1 Mustafa B. A. Djamgoz...
Articles
Journal:
Bioscience Reports
Biosci Rep (1996) 16 (1): 11–21.
Published: 01 February 1996
..., exocytosis (of β-D-N-acetylglucosaminidase) is dependent on provision of both Ca 2+ and a guanine nucleotide. In contrast, for cells permeabilised by either of the two pure proteins, a substantial extent of Ca 2+ -independent exocytosis can be elicited. When the Murex material is subject to dialysis...
Articles
Journal:
Bioscience Reports
Biosci Rep (1992) 12 (6): 495–501.
Published: 01 December 1992
... to determine minimal time requirements for exocytosis-coupled endocytosis to occur, we have analyzed by electron microscopy uptake of horserdish peroxidase (HRP) as a fluid phase marker at the onset of depolarization. We have applied rapid mixing and sampling (quenched flow) to assess events in subsecond time...
Articles
Journal:
Bioscience Reports
Biosci Rep (1990) 10 (1): 93–103.
Published: 01 February 1990
...Oliver Nüße; Manfred Lindau Exocytosis and intracellular free calcium ([Ca 2+ ] in ) were simultaneously recorded in single human neutrophils using patch-clamp capacitance measurements and the fura-2 fluorescence ratio method. Intracellular application of guanosine-5′-O(3-thiotriphosphate) (GTPγS...
Articles
Journal:
Bioscience Reports
Biosci Rep (1989) 9 (2): 243–252.
Published: 01 April 1989
... secretion exocytosis chromaffin cell calcium bradykinin angiotensin II, muscarinic Abbreviations ([Ca 2+ ] i ), cytoplasmic free calcium concentration; EGTA, ethylene glycol bis (β-amino ethyl ether)-N,N,N,N,-tetraacetic acid; Hepes, 4-(2-hydroxy ethyl)-1-piperazinethanesulphonic acid; TPA...
Articles
Journal:
Bioscience Reports
Biosci Rep (1989) 9 (1): 99–109.
Published: 01 February 1989
...P. E. R. Tatham; B. D. Gomperts ATP is not required for exocytosis from permeabilised mast cells, and therefore there is no direct role for protein phosphorylation in the late stages of the activation pathway. We have measured the timecourse of exocytosis from permeabilised cells triggered...
Articles
Journal:
Bioscience Reports
Biosci Rep (1988) 8 (5): 471–483.
Published: 01 October 1988
... the nerve terminals, the present paper demonstrates that secretion can occur to the same extent from both nerve endings and nerve swellings. 10 3 1988 © 1988 Plenum Publishing Corporation 1988 exocytosis secretion neuropeptides vasopressin neurohypophysis Bioscience Reports, Vol. 8...
Articles
Journal:
Bioscience Reports
Biosci Rep (1988) 8 (4): 335–343.
Published: 01 August 1988
...Tim Whalley; Michael Whitaker Micromolar calcium ion concentrations stimulate exocytosis in a reconstituted system made by recombining in the plasma membrane and cortical secretory granules of the sea urchin egg. The isolated cortical granules are unaffected by calcium concentrations up to 1 mM...
Articles
Journal:
Bioscience Reports
Biosci Rep (1988) 8 (1): 27–33.
Published: 01 February 1988
... transport exocytosis calcium calmodulin B-adrenergic cyclic AMP Abbreviations β-adrenergic agonist acting at its receptor cAMP, cyclic AMP; PDE, phosphodiesterase; CaM, calmodulin; Pase, phosphatase Bioscience Reports, Vol. 8, No. 1, 1988 REVIEW Defective Regulation of Apical Membrane...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 411–426.
Published: 01 May 1987
... the mechanism of stimulus-secretion. 6 5 1987 © 1987 Plenum Publishing Corporation 1987 neurosecretion nerve endings stimulus-secretion coupling secretion exocytosis Bioscience Reports, Vol. 7, No. 5, 1987 Isolated Neurosecretory Nerve Endings as a Tool for Studying the Mechanism...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 383–397.
Published: 01 May 1987
...Michael Whitaker The process of secretory granule-plasma membrane fusion can be studied in sea urchin eggs. Micromolar calcium concentrations are all that is required to bring about exocytosis in vitro . I discuss recent experiments with sea urchin eggs that concentrate on the biophysical aspects...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 399–409.
Published: 01 May 1987
... prepared from human red blood cells is minimal. © 1987 Plenum Publishing Corporation 1987 reconstitution of exocytosis exocytosis sea urchin egg plasma membrane cortical vesicles Bioscience Reports, Vol. 7, No. 5, 1987 In vitro Reconstitution of Exocytosis from Sea Urchin Egg Plasma Membrane...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 435–442.
Published: 01 May 1987
... pancreatic acinar cell cytosol of a factor which stabilizes isolated zymogen granules and inhibits cation-induced granule aggregation is outlined. The basis of this action and the significance of the cytosolic inhibitory factor in the regulation of granule mobility and exocytosis in vivo is discussed. 26...
Articles
The dual effector system for exocytosis in mast cells: Obligatory requirement for both Ca 2+ and GTP
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 369–381.
Published: 01 May 1987
... into two main sections comprising early, receptor-mediated events leading to generation of intracellular second messengers, and later events leading to membrane fusion and exocytosis. With the discovery that occupation of Ca 2+ mobilising receptors leads to activation of polyphosphoinositide...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 427–434.
Published: 01 May 1987
... in cellular functions that control endocytosis and/or exocytosis. 8 6 1987 © 1987 Plenum Publishing Corporation 1987 Ras protein membrane turnover exocytosis endocytosis Bioscience Reports, Vol. 7, No. 5, 1987 Regulation of Membrane Turnover by Ras Proteins Dafna Bar-Sagi, Anne...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 355–367.
Published: 01 May 1987
... proteins may play a role not only at the level of signal transduction coupling, but also at or near the site of exocytosis, and the mechanism by which some Botulinum toxins inhibit secretion may be associated with these proteins. 6 7 1987 © 1987 Plenum Publishing Corporation 1987 calcium...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (5): 443–454.
Published: 01 May 1987
... together with the finding that insulin secretion due to Ca 2+ or TPA is attenuated by the inhibitory guanine nucleotide GDPβS, suggest the existence of a regulatory site in exocytosis which is sensitive to guanine nucleotides. 14 7 1987 © 1987 Plenum Publishing Corporation 1987 calcium...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 251–268.
Published: 01 April 1987
...Joshua Zimmerberg Exocytosis is considered as four separate steps: adhesion, fusion/pore formation, pore widening, and content discharge. Experiments on both synthetic and natural membranes are presented to show each of these steps. Major differences are seen in the two fusing systems...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 307–311.
Published: 01 April 1987
... antitrypsin deficiency defective gene mRNA exocytosis Bioscience Reports, Vol. 7, No. 4, 1987 Alpha 1-Antitrypsin Deficiency ecretlon R. C. Foreman Received April 9, 1987 KEY WORDS: antitrypsin deficiency; defective g ne; mRNA; exocytosis. A Defect in A naturally occurring point mutation in the human...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 313–321.
Published: 01 April 1987
... dialysis for as long as 10 min. The fast “washout” phenomenon of exocytosis could be overcome by supplementing the pipette filling solution with guanosinetriphosphate (GTP) indicating a major role for GTP-binding proteins in secretion. The restoration of exocytosis was transient and decayed within three...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 269–279.
Published: 01 April 1987
... 1987 chromaffin granules docking exocytosis Bioscience Reporrs, Vol. 7, No. 4, 1987 Docking of Chromaffin Granules--A Necessary Step in Exocytosis? Theo Seh/ifer, Urs O. Karli, Felix E. Sehweizer and Max M. Burger Received April 9, 1987 KEY WORDS: chromaffin granules; docking; exocytosis...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 281–288.
Published: 01 April 1987
...-linking, assembly and interactions with secretory granule and plasma membranes. The possibility is discussed that cytoskeletal elements including the membrane-binding proteins caldesmon, p70 and p36 may be involved in granule-plasmalemmal interactions immediately prior to exocytosis. 9 4 1987...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 323–332.
Published: 01 April 1987
... © 1987 Plenum Publishing Corporation 1987 myosin light chain phosphorylation catecholamine chromaffin cell protein kinase exocytosis Abbreviations EGTA, ethyleneglycol-bis-(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; HEPES, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 333–344.
Published: 01 April 1987
... is released by the action of inositol-l,4,5 trisphosphate following stimulation of secretion. 9 4 1987 © 1987 Plenum Publishing Corporation 1987 Ca 2+ pancreas secretion exocytosis Abbreviations Used EGTA, (ethylene dioxy) diethylene-dinitrilotetraacetic acid; BAPTA, 1,2-bis...
Articles
Journal:
Bioscience Reports
Biosci Rep (1987) 7 (4): 345–353.
Published: 01 April 1987
... and calcium ionophore. Thus in both helper and cytotoxic lymphocytes, the regulated pathway of protein secretion clearly operates after triggering by the T cell antigen receptor. 9 4 1987 © 1987 Plenum Publishing Corporation 1987 lymphocyte cytotoxicity granules exocytosis Bioscience...
Articles
Journal:
Bioscience Reports
Biosci Rep (1986) 6 (10): 869–877.
Published: 01 October 1986
... in such a way that they can still interact with specific membrane receptors and they are subsequently subjected to specific cellular transport mechanisms. 21 10 1986 © 1986 Plenum Publishing Corporation 1986 pinocytosis exocytosis IgG conjugate Bioscience Reports, Vol. 6, No. 10, 1986...
