...) may accelerate the aggregation of Alzheimer’s amyloid-β peptide (Aβ 1-42 ). Here, we examined in vitro whether NQO1 and its FAD-deficient P187S mutation (NQO1*2) directly interact with Aβ 1-42 and modify its rate of aggregation. When monitored using the fluorescence of either noncovalent thioflavin T...

... a sensitive fluorescence assay based on enzyme-free and isothermal hybridization chain reaction with SYBR Green and graphene oxide (GOX) for early detection of miR-137 and miR-142, as two Alzheimer’s biomarkers. Fluorescence spectrophotometry based on SYBR Green signal and GOX as the fluorescence quencher...

...Sviatlana Shashkova; Mark C. Leake Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse technique offering exceptional contrast in conjunction with high specificity of labelling with relatively minimal perturbation to biological samples compared with many competing...

... activation of Pgp-coupled ATPase kinetics suggested a single digoxin-binding site. Using intrinsic protein fluorescence and the saturation transfer double difference (STDD) NMR techniques to probe drug–Pgp interactions, verapamil was found to have little effect on digoxin–Pgp interactions at low...

... fluorescence-based method for specific detection of DNA. The exciplex partners (5′-pyrenyl and 3′-naphthalenyl) were attached to oligonucleotides via phosphoramidate links to terminal phosphate groups. Hybridization of the probe to a complementary target in a buffer containing trifluoroethanol produced...

... and suggest that fusion proteins from different viralfamilies share common structural and functional motifs. © 2000 Plenum Publishing Corporation 2000 membrane fusion peptide–lipid interaction fluorescence viral fusion inhibitors antiviral peptides Bioscience Reports, Vol. 20, No. 6, 2000 MINI...

...Jonathan V. Rocheleau; Nils O. Petersen Receptor aggregation is believed to be an important step in the attachmentof membrane enveloped virus' to target cell membranes. A likely receptorfor Sendai virus is the ganglioside GD1a. In this work we have studied themembrane diffusion of the fluorescent...

... hexokinase. © 2000 Plenum Publishing Corporation 2000 Yeast hexokinase Stability Enzyme-micelle interaction Fluorescence Zwitterionic micelle Bioscience Reports, Vol. 20, No. 1, 2000 Increased Stability and Catalytic Efficiency of Yeast Hexokinase Upon Interaction with Zwitterionic Micelles...

...Sérgio T. Ferreira; Tatiana Coelho-Sampaio Applications of intrinsic fluorescence measurements in the study of Ca 2+ -transport ATPases are reviewed. Since the initial reports showing that the fluorescence emission was sensitive to Ca 2+ binding, a substantial amount of work has focused on the use...

... tryptophan fluorescence were followed throughout the catalytic cycle by the steady-state measurements and the stopped-flow spectrofluorometry. EDANS-fluorescence changes reflect conformational changes near the ATP binding site in the cytoplasmic domain, while tryptophan-fluorescence changes most probably...

...Dick Hoekstra; Karin Klappe The kinetics of the fusion process between erythrocyte ghosts, as induced by Sendal virus, were readily revealed by a simple fluorescence procedure previously employed to characterize the fusion of viruses with biological membranes. The method relies on the relief...

...M. V. Krishna Sastry; A. Surolia The combining region of Artocarpus integrifolia lectin has been studied by using the ligand-induced changes in the fluorescence of the lectin. The saccharide binding properties of the lectin show that C-l, C-2, C-4, and C-6 hydroxyl groups of D-galactose...