...) and incubated overnight at 37°C. Correspondence: Gabriela A. Silva ( [email protected] ) chitosan gene therapy HEK293T cells IGFBP nuclear localization signals Gene therapy entails the transfer of therapeutic genetic material into cells where the production of the encoded protein...

.... Correspondence: Jianjun Jin ( [email protected] ) 25 07 2018 09 04 2019 30 04 2019 28 05 2019 FGFR1 gastric cancer gene therapy miR-198 miRNA Gastric cancer (GC) is a prevalent cancer and a leading cause of cancer-related deaths around the globe, particularly in developing...

... transcription of genes such as p21 through the ING4-dependent association with H3K4me near the TSS [ 37 ]. aberrant expression status chromosome remodeling gene therapy inhibitor of growth 4 mechanisms non-neoplastic diseases Tumor suppressor genes (TSGs) can oppose oncogene function...

...-coding RNA microRNA oncogenesis gene therapy Altered expression of miRNAs under hypoxia may induce a pathological process via modulating the expression of their downstream genes [ 18 ]. Hypoxia-induced up-regulation of miR-301a/b increased cell autophagy and viability of prostate cancer (PCa...

... and its induction was achieved with low Dox concentrations. Remarkably, the intracellular regulatory expression of 2LTRZFP significantly inhibited HIV-1 integration and replication in HIV-inoculated SupT1 cells. This approach could provide a potential tool for gene therapy applications, which efficiently...

..., but with the development of a number of model systems and approaches, great progress is being made. Correspondence: David A. Dean ( [email protected] ) 14 09 2017 13 10 2017 17 10 2017 20 10 2017 cytoskeleton cell nucleus gene therapy intracellular transport microtubule...

... and assess whether targeting this pathway has potential for reducing neointima formation. We utilized a gene therapy approach selectively targeting cells in which the β-catenin/TCF pathway is activated by using a recombinant adenovirus Ad-TOPTK, which carries a herpes simplex virus thymidine kinase (HSV-TK...

... Attribution Licence 4.0 (CC BY) . endostatin gene therapy radiotherapy Walker-256 tumour cells were inoculated into the six-well plate at a cell density of 3 × 10 5 per well. At approximately 90% of cell confluence, Walker-256 cell lines were transfected with 5 μl PBS, 5 μg empty plasmids and 5...

... is a promising method that allows the targeting of gene therapy for heart failure. Furthermore, we found that the treated animals (liposomal magnetofection with shIGF1R) showed reduced septal and posterior wall thickness, reduced HW:BWs (heart weight-to-body weights) compared with controls. Moreover, we also...

.... © 2007 The Biochemical Society 2007 MNGIE Thymidine phosphorylase Thymidine Deoxyuridine Stem cells Gene therapy ORI GI N A L P A PE R Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE): Biochemical Features and Therapeutic Approaches M. C. Lara Æ M. L. Valentino Æ J. Torres...

... efficient in vivo gene delivery protocols. © 2006 Springer Science+Business Media, LLC 2006 Cationic lipid Cell uptake Endocytosis Gene therapy Gene delivery Nuclear import Plasmid DNA Stability Transfection Abstract Cationic lipids are conceptually and methodologically simple tools...

...-mediated transposition as a tool for gene transfer to chromosomes and ultimately for human gene therapy. Transfection of mouse 3T3 cells, HeLa cells, and human A549 lung carcinoma cells with a transposon containing the neomycin phosphotransferase ( NEO ) gene resulted in a several-fold increase in drug...

... Corporation 2002 Liposomes non-viral delivery systemic delivery gene therapy optimization Bioscience Reports, Vol. 22, No. 2, April 2002 ( 2002) MINI REVIEW Cationic Liposome-mediated Gene Delivery In vivo Nancy Smyth Templeton Receiûed Noûember 1, 2001 Several improvements have been made...

... attention to polyunsaturated lipids, membrane proteins and ion channels, surfactant additives in membranes, and lipid–DNA gene transfer complexes. © 2002 Plenum Publishing Corporation 2002 Biomembranes membrane proteins lipid–DNA complexes lipid–protein interactions gene therapy polyunsaturated...

... showed significantly less cytotoxicity compared to the commonly used cationic lipsomes or PEI mediated transfection and several cell lines were transfected with high efficiency. LPDII vectors avoid the use of toxic cationic lipids and may have potential application in gene therapy. © 2000 Plenum...