... change as revealed by CD, tryptophan fluorescence, and trypsin digestion. The Zn 2+ -induced conformational change was required for the formation of GAPR-1 oligomers and amyloid-like assemblies in the presence of heparin, as shown by ThT fluorescence and TEM. Molecular dynamics simulations show binding...

... and conserved in vertebrates. We used recombinant derivatives of RPSA and its N- and C-domains to quantify its interactions with ligands by in-vitro immunochemical and spectrofluorimetric methods. Both N- and C-domains bound laminin with K D (dissociation constants) of 300 nM. Heparin bound only to the N-domain...

...Wenjing Zhao; Scott A. McCallum; Zhongping Xiao; Fuming Zhang; Robert J. Linhardt Heparin and HS (heparan sulfate) exert their wide range of biological activities by interacting with extracellular protein ligands. Among these important protein ligands are various angiogenic growth factors...