... mechanism and lipid sensitivity of each PKN isoform using full-length enzymes and synthetic peptide substrate. Steady-state kinetic analysis revealed that PKN1–3 follows a sequential ordered Bi–Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism...

... homodimer. Owing to these differences, here we examine the kinetic mechanism of soybean ATP sulfurylase [GmATPS1 ( Glycine max (soybean) ATP sulfurylase isoform 1)]. For the forward reaction (APS synthesis), initial velocity methods indicate a single-displacement mechanism. Dead-end inhibition studies...

... differing from its conventional metabolism. A simple kinetic mechanism is considered, in which IP 3 -induced Ca 2+ channel opening is followed by the step of IP 3 conversion and channel closure. Examination of the resulting mathematical model shows that it can reproduce well both partial release of stored...