... transporters. Reconstitution into model membranes, such as liposomes, is a suitable approach to study lipid transporters in vitro and to investigate their key molecular features. In this review, we discuss the current approaches for reconstituting ATP-driven lipid transporters into large liposomes and common...

.... In the accompanying article, Horiuchi et al. ( Biosci. Rep. (2019) 39(4) , BSR20190213) evaluate the availability of apolipoprotein B-depleted serum for CEC assays. Using their recently developed immobilized liposome-bound gel beads (ILG) method, Horiuchi et al. demonstrate that apolipoprotein B-depleted serum...

...Yong Serk Park Tumor-specific targeting is a critical goal in the research area of liposomal drug delivery. Identification of the specific interactions between ligands and target tumor cells is a principle prerequisite in achieving this goal. Generally, tumor cells aberrantly express tumor...

...Nancy Smyth Templeton Several improvements have been made in liposomal delivery, thus making this technology potentially useful for treatment of certain diseases in the clinic. Success in non-viral delivery is complicated and requires optimization of several components. These components include...

...Tatsuhiro Ishida; Hideyoshi Harashima; Hiroshi Kiwada The clearance rate of liposomal drugs from the circulation is determined by the rate and extent of both drug release and uptake of liposomes by cells of the mononuclear phagocyte system (MPS). Intravenously injected liposomes initially come...

... to the cytosol of DCs. We present in vitro and pre-clinical studies reporting specific immunity to viral, parasitic infection and tumor growth. © 2002 Plenum Publishing Corporation 2002 Liposomes dendritic cells cytotoxic T lymphocyte vaccine Bioscience Reports, Vol. 22, No. 2, April 2002 ( 2002...

... envelopes (virosomes), liposomes, and immune-stimulating complexes (ISCOMs). We will concentrate on delivery of the protein antigen ovalbumin (OVA) since extensive studies with this antigen have been performed for all of the systems discussed, allowing direct comparison of antigen delivery efficiency...

..., the experimental evidence is reviewed, and discussed in terms of the underlying molecular mechanisms of fusion, and of the possible physiological relevance of these findings. KEY WORDS: Phospholipase C; sphingomyelinase; membrane fusion; liposomes; model membranes; non-bilayer lipids; non-lamellar lipid phase...

...Wenjin Guo; Robert J. Lee Synthetic gene transfer vectors based on polyplexes complexed to anionic liposomes (LPDII vectors) were characterized for their transfection efficiency in cultured mammalian cells. The effects of polycation to DNA ratio, lipid to DNA ratio, choice of polycation and lipid...

...Robert J. Lee; Susan Wang; Mary Jo Turk; Philip S. Low Targeted liposomal drug formulations may enter cells by receptor-mediated endocytosis and then traffick by membrane flow into acidic intracellular compartments. In order to understand the impact of these intracellular pH changes on liposomal...

... and lectin-affinity on wheat germ agglutinin. Following exchange of Triton X-100 with CHAPS or n-octylglucoside, the purified receptor was incorporated into egg phosphatidylcholine liposomes upon, detergent removal by dialysis (lipid/protein ratio 15:1 to 45:1 (w/w) Reconstitution of the receptor...

...F. Severcan; S. Cannistraro The effect of α -tocopherol (αT) on partitioning and fluidity changes occurring in phospholipid liposomes have been investigated by monitoring the X-band ESR spectrum of the high resolution amphiphilic spin probe perdeutero-di-t-butyl nitroxide (PDDTBN), which partitions...

...L. Finch; W. M. Southerland Bisulfite has been shown to induce leakage of encapsulated substances from liposomal vesicles. The bisulfite induced leakage of either DNP-tyrosine, potassium ferricyanide, or [ 3 H]glycine was observed to be greater with lipsomes composed of phospholipids containing...

...Nirmalendu Das; Mukul Kumar Basu; Manoj Kumar Das 125 Iodine labelled human immunoglobulin-G encapsulated liposomes were administered orally to rats. Distribution of radioactivity was checked in various tissues and in portal blood. The effect of dextran sulfate (DS 500,000 m. wt., liver blockade...