... of these writers and readers during the process of cell proliferation is critical for our understanding of their role in carcinogenesis. Here, we immunopurified each of these proteins and used mass spectrometry to define their associated non-histone proteins, individually and at two different phases of the cell...

... BY) . Aristaless-related homeobox Cortex development Mass Spectrometry phosphorylation Post-translational modification of proteins is an essential cellular process that influences the protein localization, function, and degradation. Phosphorylation is one of the most common modifications that can take...

... reaction. The product of this reaction can be selectively detected and quantified with high sensitivity using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform. Further, using this method, we capture and quantify the unique acyl chain composition of PI5P from Drosophila cells and tissues...

... (CC BY) . amino acid metabolism mass spectrometry parasitic protozoa trypanosomes T. b. brucei strain 427 was grown in CMM + 10% FBS Gold (PAA, Piscataway, NJ) at 37°C, 5% CO 2 for U- 13 C-labelled tracking. For l -methionine studies, a 1:1 mix of unlabelled and U- 13 C l...

...-Chao Kidney stone mass spectrometry metabolomics Oxalate crystal Nephrolithiasis is a highly prevalent disease worldwide with rates ranging from 1 to 13% [ 1 ]. The prevalence is progressively rising due to various factors including metabolic dysfunction [ 2 , 3 ] and diet [ 4...

... forms of AKT are present in melanoma cells nuclei. Using mass spectrometry in combination with protein-crosslinking and co-immunoprecipitation, we identified a series of putative protein partners of nuclear AKT, including heterogeneous nuclear ribonucleoprotein (hnRNP), cytoskeleton proteins β-actin, γ...

... region in photoreceptors. In summary, hPLD1 likely represents the ancestral PLD in mammalian genomes while hPLD2 represents neofunctionalization to generate PA at distinct subcellular membranes. Drosophila lipids membranes mass spectrometry phospholipase D Correspondence: Padinjat...

... Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . calcareous corpuscles host proteins Mass spectrometry metabolically labeled proteins Taenia crassiceps Taenia solium During the last decades, considerable...

... in pET28c vector and transformed in E. coli strain DH5α for screening of mutant clones. enzyme kinetics mass spectrometry molecular dynamic simulations phosphorylation/dephosphorylation site-directed mutagenesis Nucleotide metabolism is a crucial process in all spheres of life...

... their influence on nerve cell growth. Unusual results compelled us to analyse the composition and purity of both preparations of these proteoglycans (PGs) using both mass spectrometry (MS) and Western blotting, with and without various enzymatic deglycosylations. Commercial ‘decorin’ and ‘biglycan’ were found...

...Monika Kijewska; Karolina Radziszewska; Marta Cal; Mateusz Waliczek; Piotr Stefanowicz; Zbigniew Szewczuk The combination of deuterium–hydrogen exchange (DHX) and mass spectrometry (MS) can be used for studying a high pressure denaturation (HPD) of proteins. Herein we present the results...

.... carboxypeptidase A cardiovascular disease chymase mass spectrometry mast cell truncated apolipoprotein A-I MCs (mast cells) distribute to mucosal surfaces and skin throughout the body and play an important role in the first line of defence against pathogens. When activated, MCs release granules...

...) flow cytometry human umbilical-vein endothelial cell (HUVEC) in vitro mass spectrometry Significant positive expression of four antigens on HUVEC was determined through flow cytometry and was compared between the serum donors. CD31, CD54 and CD106 demonstrated significant differences...

...Ida Chiara Guerrera; Oliver Kleiner Mass spectrometry has arguably become the core technology in proteomics. The application of mass spectrometry based techniques for the qualitative and quantitative analysis of global proteome samples derived from complex mixtures has had a big impact...

... Springer 2005 Functional proteomics mass spectrometry protein–protein interactions affinity-based strategies Abbreviations 1D-gel, monodimensional gel electrophoresis; 2D-gel, two-dimensional gel electrophoresis; AldA-NRE, Aldolase A negative regulatory element; ES-LC-MS/MS...

... folate folinic acid thymic extract sheep thymus thymocytes mass spectrometry Bioscience Reports, Vol. I1, No. 2, 1991 Identification of a Mammalian Growth Factor as a Ribofolate Peptide Ul f Ernst r6m 1 Received April 5, 1991 Thymocyte growth peptide (TGP) promotes DNA synthesis of immature...