...Alice Cassiani; Paul G. Furtmüller; Marco Borsari; Gianantonio Battistuzzi; Stefan Hofbauer In this present study, we investigated the H 2 O 2 -induced oligomerization of wild-type human neuroglobin (hNgb) and of some selected variants (C46AC55A, Y44A, Y44F, Y44AC46AC55A, Y44AC46AC55A) to clarify...

... progesterone binding. Here, we used small-angle X-ray scattering (SAXS) to investigate the influence of ligand binding and oxidation on apoD oligomerisation and conformation. As a solution-based technique, SAXS is well suited to detect changes in oligomeric state and conformation in response to ligand binding...

... of oligomeric states and indeed, it is known that the oligomerization state of a GPCR can modulate its pharmacology and function. A number of experimental techniques have been devised to study GPCR oligomerization including those based upon traditional biochemistry such as blue-native PAGE (BN-PAGE), co...

... is unable to interact with self or with wt hENT1. Based on these data we propose that glycosylation at N48 is critical for the localization, function and oligomerization of hENT1. Full-length hENT1 conjugated with a 3xFLAG tag in a pCDNA 3.1 vector was used as the template and point mutations were...

... expressing controlled combinations of VASP, Mena and EVL, we evaluated the composition of Ena/VASP oligomers and found that VASP forms oligomers without apparent bias with itself, Mena or EVL. However, Mena and EVL showed only weak hetero-oligomerization, suggesting specificity in the association of Ena/VASP...

..., attributable to reduced turnover of hRFC transcripts. hRFC homo-oligomerization was unaffected by increasing LCV. Cell surface hRFC paralleled [ 3 H]methotrexate transport and increased from 0.5 to 2 nM LCV, and then decreased (~2-fold) with increasing LCV up to 20 nM. hRFC was localized to the cell surface...

... and regulation, however, are little understood. It has a unique N-terminal domain and CTD (C-terminal domain) flanking a conserved Sirtuin catalytic core and these extensions are assumed to mediate Sirt1-specific features such as homo-oligomerization and activation by resveratrol. To analyse the architecture...

... + . Collectively, these data strongly suggest the presence of an extracellular redox system capable of converting adiponectin oligomers. adipocyte adiponectin oligomerization oxidation–reduction redox thiol Adiponectin, an abundant serum protein secreted mainly from adipocytes with anti-inflammatory...

... email [email protected] 18 11 2009 6 1 2010 15 1 2010 channel opening membrane protein integration oligomerization protein translocation translocon ribosome binding Protein translocation across the ER (endoplasmic reticulum) membrane in eukaryotes...

... role of ORF-C4 as a small chaperone which is involved in the response to stress (including encystation) in G. lamblia . © The Authors Journal compilation © 2009 Biochemical Society 2009 α-crystallin domain chaperone oligomerization parasite small heat-shock protein (sHsp) stress...

... fusion viral glycoprotein heptad repeats proteolytic processing glycosylation oligomerization fusion peptides Bioscience Reports, Vol. 20, No. 6, 2000 MINI REVIEW Virus Membrane Fusion Proteins: Biological Machines that Undergo a Metamorphosis Rebecca Ellis Dutch,1 Theodore S. Jardetzky,2...