... License 4.0 (CC BY) . Cytochrome P450 Escherichia coli Pichia pastoris recombinant protein Saccharomyces cerevisiae Figure 1 Exemplary reaction catalyzed by a P450 11-desoxycorticosterone is hydroxylated at position 11 by CYP11B1 leading to corticosterone. However...

... NbAahII10 C/S, 6 litres of Terrific Broth supplemented with 0.1% (w/v) glucose and 100 μg/ml ampicillin was grown at 37°C until an A 600nm between 0.6 and 0.9 was reached. Expression of the recombinant protein was induced with 1 mM isopropyl β- D -thiogalactoside (Sigma Aldrich) and the culture...

.... 23 6 1990 © 1990 Plenum Publishing Corporation 1990 Proenkephalin A recombinant protein E. coli expression vector DNA Bioscience Reports, Vol. 10, No. 5, 1990 Expression of a Human Proenkephalin A cDNA in Escherichia coli Hans-Jiirg Monstein Received June 23, 1990 A 1000 base...