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Keywords: solubilising agents
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Articles
Journal:
Bioscience Reports
Biosci Rep (2023) 43 (10): BSR-2022-2258_COR.
Published: 20 October 2023
... The Author(s). 2023 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . glycerol kinase sarkosyl solubilising agents Type 2 diabetes mellitus The authors...
Articles
Journal:
Bioscience Reports
Biosci Rep (2023) 43 (4): BSR20222258.
Published: 21 April 2023
... mechanisms and structure of the human GK are unknown. Methods: The human GK gene was cloned into the pET-24a(+) vector and over-expressed in Escherichia coli BL21 (DE3). Since the protein was expressed as inclusion bodies (IBs), various culture parameters and solubilising agents were used but they did...
Includes: Supplementary data
