High levels of high-density lipoprotein (HDL) cholesterol have been reported to protect against the development of atherosclerosis in humans by increasing reverse cholesterol transport and inhibiting the oxidation of low-density lipoprotein (LDL) due to the paraoxonase content of HDL. The purpose of the present study was to assess if there are any relationships between in vivo increases in serum levels of immunological LDL oxidation markers [autoantibodies against oxidized LDL, autoantibodies against malondialdehyde-modified LDL, LDL immune complexes and anti-cardiolipin autoantibodies], paraoxonase activity and the development of atherosclerosis in control rabbits and in transgenic rabbits expressing human apolipoprotein (apo) A-I. A total of 13 apo A-I transgenic rabbits and 18 non-transgenic littermates were fed on a cholesterol-rich diet (0.4%, w/w) for 14 weeks, and were monitored at weeks 0, 2, 6, 10 and 14. Aortic atherosclerotic lesions were measured at the end of this period. Human apo A-I transgenic rabbits with high HDL cholesterol levels were not protected against the development of atherosclerosis when they were fed on a cholesterol-rich diet which induced dramatic hypercholesterolaemia. Immunological markers of LDL oxidation increased and serum paraoxonase activity decreased similarly in control and transgenic rabbits. In conclusion, the present study demonstrates that high HDL cholesterol levels are ineffective in inhibiting increases in immunological markers of LDL oxidation and the development of atherosclerosis in a mammal with severe hypercholesterolaemia.
Skip Nav Destination
Article navigation
Research Article|
February 13 2001
Increased levels of high-density lipoprotein cholesterol are ineffective in inhibiting the development of immune responses to oxidized low-density lipoprotein and atherosclerosis in transgenic rabbits expressing human apolipoprotein (apo) A-I with severe hypercholesterolaemia
Agnès BOULLIER;
Agnès BOULLIER
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Nathalie HENNUYER;
Nathalie HENNUYER
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Anne TAILLEUX;
Anne TAILLEUX
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Christophe FURMAN;
Christophe FURMAN
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Nicolas DUVERGER;
Nicolas DUVERGER
†Aventis, Center of Research, Vitry-Alfortville, Vitry sur Seine, France
Search for other works by this author on:
Jean-Michel CAILLAUD;
Jean-Michel CAILLAUD
†Aventis, Center of Research, Vitry-Alfortville, Vitry sur Seine, France
Search for other works by this author on:
Graciella CASTRO;
Graciella CASTRO
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Catherine FIEVET;
Catherine FIEVET
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Jean-Charles FRUCHART;
Jean-Charles FRUCHART
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Search for other works by this author on:
Patrick DURIEZ
*Department of Atherosclerosis, Pasteur Institute, INSERM U325 and University of Lille II, Lille, France
Correspondence: Dr Patrick Duriez, Département d'Athérosclérose, INSERM U325, Institut Pasteur, 1, rue du Professeur Calmette, BP 245, 59019 Lille cedex, France (e-mail [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 03 2000
Revision Received:
October 16 2000
Accepted:
December 11 2000
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2001
2001
Clin Sci (Lond) (2001) 100 (3): 343–355.
Article history
Received:
August 03 2000
Revision Received:
October 16 2000
Accepted:
December 11 2000
Citation
Agnès BOULLIER, Nathalie HENNUYER, Anne TAILLEUX, Christophe FURMAN, Nicolas DUVERGER, Jean-Michel CAILLAUD, Graciella CASTRO, Catherine FIEVET, Jean-Charles FRUCHART, Patrick DURIEZ; Increased levels of high-density lipoprotein cholesterol are ineffective in inhibiting the development of immune responses to oxidized low-density lipoprotein and atherosclerosis in transgenic rabbits expressing human apolipoprotein (apo) A-I with severe hypercholesterolaemia. Clin Sci (Lond) 1 March 2001; 100 (3): 343–355. doi: https://doi.org/10.1042/cs1000343
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |