In order to test the proposal that the aldosterone specificity of mineralocorticoid receptors in the collecting duct depends on inactivation of glucocorticoids by the enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD), we have assessed the effect of pharmacological inhibition of 11β-HSD on collecting duct Na+ reabsorption in vivo. Adrenalectomized rats (n = 14) were infused intravenously with high-dose corticosterone, and late-distal tubules were perfused orthogradely with artificial tubular fluid containing [14C]inulin and 22Na; urinary recoveries of the radioisotopes were monitored. Half of the rats received intravenous carbenoxolone to inhibit renal 11β-HSD activity. The urinary recovery of [14C]inulin was complete in both groups of animals (101ŷ2% versus 101ŷ3%), but the recovery of 22Na was lower in carbenoxolone-treated rats (34ŷ5%) than in the corticosterone-alone group (54ŷ4%, P < 0.01). These data, which provide the first demonstration of enhanced Na+ reabsorption in the distal nephron during inhibition of renal 11β-HSD in vivo, strongly support the proposal that 11β-HSD normally prevents endogenous glucocorticoid from exerting mineralocorticoid-like effects.
Skip Nav Destination
Article navigation
Rapid Communication|
July 25 2001
RAPID COMMUNICATIONIn vivo inhibition of renal 11β-hydroxysteroid dehydrogenase in the rat stimulates collecting duct sodium reabsorption
M. A. BAILEY;
M. A. BAILEY
1Centre for Nephrology, Institute of Urology and Nephrology, Middlesex Hospital, Mortimer Street, London W1N 8AA, U.K.
Search for other works by this author on:
R. J. UNWIN;
R. J. UNWIN
1Centre for Nephrology, Institute of Urology and Nephrology, Middlesex Hospital, Mortimer Street, London W1N 8AA, U.K.
Search for other works by this author on:
D. G. SHIRLEY
1Centre for Nephrology, Institute of Urology and Nephrology, Middlesex Hospital, Mortimer Street, London W1N 8AA, U.K.
Correspondence: Dr D. G. Shirley ([email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 23 2001
Revision Received:
May 18 2001
Accepted:
June 11 2001
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2001
2001
Clin Sci (Lond) (2001) 101 (2): 195–198.
Article history
Received:
March 23 2001
Revision Received:
May 18 2001
Accepted:
June 11 2001
Citation
M. A. BAILEY, R. J. UNWIN, D. G. SHIRLEY; RAPID COMMUNICATIONIn vivo inhibition of renal 11β-hydroxysteroid dehydrogenase in the rat stimulates collecting duct sodium reabsorption. Clin Sci (Lond) 1 August 2001; 101 (2): 195–198. doi: https://doi.org/10.1042/cs1010195
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |