The aim of this study was to compare the leptin responses to a high-fat meal in lean and obese men, and to investigate whether the net leptin response (area under the incremental curve) after the meal was related to the thermic effect of food (TEF). Blood samples were collected after an overnight fast and every 2h for 8h after a high-fat breakfast (60g of fat/m2 body surface area) in 12 lean and 12 obese men for determination of glucose, insulin and leptin. The TEF was calculated as postprandial energy expenditure minus fasting energy expenditure, as measured by indirect calorimetry. Fasting plasma glucose levels were similar in lean and obese men, and increased in the same way after the meal. Fasting and postprandial plasma insulin concentrations were significantly greater in obese than in lean men (P < 0.01 and P < 0.05 respectively). Accordingly, obese men showed a significantly higher net insulin response than lean subjects (P < 0.001). Fasting plasma leptin levels were greater in obese than in lean men (P < 0.001). After the meal, plasma leptin increased significantly in lean men, whereas it decreased in obese men (group by time interaction, P < 0.01). The net response of leptin was greater in lean than in obese men, but this did not reach statistical significance (P = 0.07). Moreover, the TEF was similar in the two groups. No significant relationship was observed between either the net insulin response or the net leptin response after the high-fat meal and the TEF of lean subjects (-0.05 < r < 0.31). In obese men, the net response of insulin was correlated significantly with TEF (r = 0.70, P < 0.05), whereas the net response of leptin was not (r = -0.40). These results suggest that obesity is related to an impaired regulation of leptin by insulin, since leptin levels increased in lean men but decreased in obese men following a high-fat meal. Moreover, the fact that the postprandial leptin responses of both lean and obese men were not significantly related to the TEF suggests that the ob gene product is probably not acutely involved in the control of this energy expenditure component in humans.
Skip Nav Destination
Article navigation
Research Article|
August 29 2001
Difference in leptin response to a high-fat meal between lean and obese men
P. IMBEAULT;
P. IMBEAULT
*Division of Kinesiology, Department of Social and Preventive Medicine, Laval University, Ste-Foy, Québec, Canada G1K 7P4
Search for other works by this author on:
E. DOUCET;
E. DOUCET
*Division of Kinesiology, Department of Social and Preventive Medicine, Laval University, Ste-Foy, Québec, Canada G1K 7P4
Search for other works by this author on:
P. MAURIÈGE;
P. MAURIÈGE
*Division of Kinesiology, Department of Social and Preventive Medicine, Laval University, Ste-Foy, Québec, Canada G1K 7P4
†Lipid Research Center, Laval University Medical Research Center, Ste-Foy, Québec, Canada G1V 4G2
Search for other works by this author on:
S. ST-PIERRE;
S. ST-PIERRE
*Division of Kinesiology, Department of Social and Preventive Medicine, Laval University, Ste-Foy, Québec, Canada G1K 7P4
Search for other works by this author on:
C. COUILLARD;
C. COUILLARD
†Lipid Research Center, Laval University Medical Research Center, Ste-Foy, Québec, Canada G1V 4G2
Search for other works by this author on:
N. ALMÉRAS;
N. ALMÉRAS
†Lipid Research Center, Laval University Medical Research Center, Ste-Foy, Québec, Canada G1V 4G2
Search for other works by this author on:
J.-P. DESPRÉS;
J.-P. DESPRÉS
†Lipid Research Center, Laval University Medical Research Center, Ste-Foy, Québec, Canada G1V 4G2
‡Québec Heart Institute, Laval Hospital Research Center, Ste-Foy, Québec, Canada, G1V 4G5
Search for other works by this author on:
A. TREMBLAY
*Division of Kinesiology, Department of Social and Preventive Medicine, Laval University, Ste-Foy, Québec, Canada G1K 7P4
Correspondence: Dr Angelo Tremblay (e-mail [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
January 16 2001
Revision Received:
April 30 2001
Accepted:
June 11 2001
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2001
2001
Clin Sci (Lond) (2001) 101 (4): 359–365.
Article history
Received:
January 16 2001
Revision Received:
April 30 2001
Accepted:
June 11 2001
Citation
P. IMBEAULT, E. DOUCET, P. MAURIÈGE, S. ST-PIERRE, C. COUILLARD, N. ALMÉRAS, J.-P. DESPRÉS, A. TREMBLAY; Difference in leptin response to a high-fat meal between lean and obese men. Clin Sci (Lond) 1 October 2001; 101 (4): 359–365. doi: https://doi.org/10.1042/cs1010359
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |