The aim of the present study was to assess the inotropic effects of vasoactive intestinal peptide (VIP) on isolated myocardial trabeculae from the right atrium and the left ventricle of human hearts. Furthermore, using reverse transcriptase-PCR, we wanted to determine the presence of mRNAs encoding the three cloned human VIP receptors, VPAC1, VPAC2 and PAC1. The trabeculae were paced at 1.0Hz in tissue baths, and changes in isometric contractile force upon exposure to agonist were studied. VIP had a potent positive inotropic effect in some of the atrial and ventricular trabeculae tested. This effect was almost completely blocked by the VIP-receptor antagonist VIP-(6-28). mRNAs encoding the human VPAC1, VPAC2 and PAC1 receptors were detected in human myocardial trabeculae from both the right atrium and the left ventricle. In conclusion, VIP has a direct positive inotropic effect in both the atria and the ventricles of the human heart. The presence of mRNAs for the VPAC1, VPAC2 and PAC1 receptors suggest that VIP may mediate its effect via these receptors.
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Research Article|
November 20 2001
Vasoactive intestinal peptide has a direct positive inotropic effect on isolated human myocardial trabeculae
Ole SAETRUM OPGAARD;
*Department of Pharmacology, Erasmus University, 3000 DR Rotterdam, The Netherlands
†Department of Medicine, University Hospital, 22185 Lund, Sweden
Correspondence: Dr Ole Saetrum Opgaard, Department of Pharmacology, College of Medicine, 360 Med Surge II, University of California, Irvine, CA 92697-4625, U.S.A. (e-mail [email protected]).
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Mikael KNUTSSON;
Mikael KNUTSSON
†Department of Medicine, University Hospital, 22185 Lund, Sweden
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René DE VRIES;
René DE VRIES
*Department of Pharmacology, Erasmus University, 3000 DR Rotterdam, The Netherlands
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Beril TOM;
Beril TOM
*Department of Pharmacology, Erasmus University, 3000 DR Rotterdam, The Netherlands
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Pramod R. SAXENA;
Pramod R. SAXENA
*Department of Pharmacology, Erasmus University, 3000 DR Rotterdam, The Netherlands
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Lars EDVINSSON
Lars EDVINSSON
†Department of Medicine, University Hospital, 22185 Lund, Sweden
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Publisher: Portland Press Ltd
Received:
March 07 2001
Revision Received:
June 04 2001
Accepted:
July 13 2001
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2001
2001
Clin Sci (Lond) (2001) 101 (6): 637–643.
Article history
Received:
March 07 2001
Revision Received:
June 04 2001
Accepted:
July 13 2001
Citation
Ole SAETRUM OPGAARD, Mikael KNUTSSON, René DE VRIES, Beril TOM, Pramod R. SAXENA, Lars EDVINSSON; Vasoactive intestinal peptide has a direct positive inotropic effect on isolated human myocardial trabeculae. Clin Sci (Lond) 1 December 2001; 101 (6): 637–643. doi: https://doi.org/10.1042/cs1010637
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