Cholecystokinin (CCK) release after a standard test meal is decreased in coeliac patients. The aim of the study was to determine the origin of the CCK deficiency and the relationship between the distinctive types of mucosal lesions observed in coeliac disease and the number of duodenal CCK cells and their peptide and mRNA content. Duodenal biopsies were obtained in ten controls and nineteen coeliac patients [seven with atrophic mucosa, six with increased numbers of intraepithelial lymphocytes (IELs) and six with fully normalized mucosa]. Immunocytochemistry was performed with a CCK C-terminal-specific polyclonal antiserum. The CCK cells were counted and related to the epithelial area using a semi-automated image analyser. CCK content in mucosal extracts was determined by radioimmunoassay. mRNA was measured with a semi-quantitative reverse transcriptase–PCR method using specific CCK and ribosomal protein L19 (RPL19) primers. CCK tissue concentration and CCK mRNA were significantly reduced in patients with atrophic mucosa [12.2 (range 6.9–17.5) pmol/g; CCK/RPL19 ratio 0.64 (0.30–0.99)] compared with patients with normal mucosa [40.5 (30.4–50.7) pmol/g; CCK/RPL19 ratio 1.40 (0.41–2.40)] or controls [42.7 (18.2–67.2) pmol/g; CCK/RPL19 ratio 1.35 (1.09–1.62)]. A similar decrease was observed in patients with an excess of IELs, 13.9 (3.8–31.8) pmol/g and 0.86 (0.57–1.15) pmol/g respectively. The number of CCK cells was, however, similar in all groups. Duodenal CCK concentration and mRNA are decreased not only in the mucosa presenting atrophic changes but also when disease activity is limited to infiltration by IELs. Reduced expression of the CCK gene could therefore be related to suppressive factors induced by the inflammatory infiltrate.
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Research Article|
July 19 2002
Expression of cholecystokinin in the duodenum of patients with coeliac disease: respective role of atrophy and lymphocytic infiltration
Pierre H. DEPREZ;
*Department of Gastroenterology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
Correspondence: Dr Pierre H. Deprez (e-mail [email protected]).
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Christine SEMPOUX;
Christine SEMPOUX
†Department of Pathology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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Christine DE SAEGER;
Christine DE SAEGER
*Department of Gastroenterology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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Jacques RAHIER;
Jacques RAHIER
†Department of Pathology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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Paul MAINGUET;
Paul MAINGUET
*Department of Gastroenterology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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Stanislas PAUWELS;
Stanislas PAUWELS
‡Department of Nuclear Medicine, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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André GEUBEL
André GEUBEL
*Department of Gastroenterology, Cliniques Universitaires St-Luc, Catholic University of Louvain, Ave Hippocrate 10, B-1200 Brussels, Belgium
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Publisher: Portland Press Ltd
Received:
January 30 2002
Accepted:
April 29 2002
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2002
2002
Clin Sci (Lond) (2002) 103 (2): 171–177.
Article history
Received:
January 30 2002
Accepted:
April 29 2002
Citation
Pierre H. DEPREZ, Christine SEMPOUX, Christine DE SAEGER, Jacques RAHIER, Paul MAINGUET, Stanislas PAUWELS, André GEUBEL; Expression of cholecystokinin in the duodenum of patients with coeliac disease: respective role of atrophy and lymphocytic infiltration. Clin Sci (Lond) 1 August 2002; 103 (2): 171–177. doi: https://doi.org/10.1042/cs1030171
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