In bronchoalveolar lavage (BAL) fluid from ventilated patients, cytotoxic oxidant activity is correlated with neutrophil activation. The aim of the present study was to investigate the hypothesis that BAL fluid induces activation of the transcription nuclear factor-κB (NF-κB) in human alveolar cells, in correlation with inflammatory mediators. We measured endotoxin, inflammatory cytokines [interleukin-1β (IL-1β), IL-8], nitrated proteins and the activity of myeloperoxidase (MPO) in BAL fluid from ventilated patients developing bronchopneumonia (n = 19 samples) or with acute respiratory distress syndrome (ARDS) (n = 14), and from ARDS/infection-free patients (n = 11). We also exposed alveolar cells to the BAL fluid or to human MPO, H2O2 or HOCl, and tested nuclear extracts for the activation of NF-κB. IL-1β, IL-8, nitrated protein, MPO and endotoxin levels were significantly higher in BAL fluid from patients with bronchopneumonia than in that from the ARDS and ARDS/infection-free groups. A correlation was observed between IL-8 and MPO values (r = 0.82). The level of NF-κB activity induced by the BAL fluid was correlated with levels of IL-1β (P<0.001), IL-8 (P<0.005) and MPO (P<0.002), and with the neutrophil count (P<0.002), and was higher for BAL fluid from the bronchopneumonia group. NF-κB activation by MPO was also demonstrated. The activation of NF-κB by BAL fluid, especially that from bronchopneumonia patients, suggests that a similar phenomenon may occur in vivo, leading to potential amplification of the inflammatory reaction.
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November 14 2002
Bronchoalveolar lavage fluids of patients with lung injury activate the transcription factor nuclear factor-κB in an alveolar cell line
M. NYS;
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
†Center of Oxygen Research and Development, University of Liége, Liége, Belgium
Correspondence: Dr Monique Nys (e-mail [email protected]).
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G. DEBY-DUPONT;
G. DEBY-DUPONT
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
†Center of Oxygen Research and Development, University of Liége, Liége, Belgium
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Y. HABRAKEN;
Y. HABRAKEN
‡Laboratory of Fundamental Virology, University of Liége, Liége, Belgium
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S. LEGRAND-POELS;
S. LEGRAND-POELS
‡Laboratory of Fundamental Virology, University of Liége, Liége, Belgium
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D. LEDOUX;
D. LEDOUX
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
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J.-L. CANIVET;
J.-L. CANIVET
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
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P. DAMAS;
P. DAMAS
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
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M. LAMY
M. LAMY
*Department of Anaesthesia and Intensive Care, CHU de Liége, B35, B4000, Sart Tilman, Liége, Belgium
†Center of Oxygen Research and Development, University of Liége, Liége, Belgium
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Publisher: Portland Press Ltd
Received:
May 17 2002
Revision Received:
July 17 2002
Accepted:
September 20 2002
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2002
2002
Clin Sci (Lond) (2002) 103 (6): 577–585.
Article history
Received:
May 17 2002
Revision Received:
July 17 2002
Accepted:
September 20 2002
Citation
M. NYS, G. DEBY-DUPONT, Y. HABRAKEN, S. LEGRAND-POELS, D. LEDOUX, J.-L. CANIVET, P. DAMAS, M. LAMY; Bronchoalveolar lavage fluids of patients with lung injury activate the transcription factor nuclear factor-κB in an alveolar cell line. Clin Sci (Lond) 1 December 2002; 103 (6): 577–585. doi: https://doi.org/10.1042/cs1030577
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