In an attempt to understand the significance of endothelin-1 (ET-1) and vasoactive intestinal contractor (VIC)/ET-2 peptides in organs during perinatal development, we performed quantitative analysis of ET-1 and VIC gene expression in mouse organs obtained from embryos at days 14 and 17 (E-14 and E-17) of pregnancy, neonates at days 0, 1, 3 and 7 after birth (N-0, -1, -3 and -7), and adult mice (10 weeks old). In intestine, VIC gene expression progressively increased between E-14 and N-1 (approximately 10-fold) and then remained constant into adulthood. ET-1 gene expression exhibited a one-step increase between E-17 and N-0, subsequently remaining constant. In lung, a sharp increase in ET-1 mRNA level (approximately 10-fold) was noticed between E-14 and N-0. The gene expression pattern of VIC, with a peak at N-0, was similar to that of ET-1 although the expression level of VIC was two to three orders of magnitudes lower than that of ET-1. Gene expression patterns of ET-1 and VIC remained nearly constant in brain, heart, liver and kidney throughout the period examined. Considering that the intestinal and pulmonary gene expression levels of both genes reached almost the same level as observed in adult soon after birth, we suggest that these peptides may be involved in the emergence and maintenance of intestinal and pulmonary functions vital after birth.
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Conference Article|
September 01 2002
Expression of endothelin-1 and vasoactive intestinal contractor genes in mouse organs during the perinatal period
Tsuyoshi UCHIDE
;
*
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 35-1, Higashi 23-bancho, Towada, Aomori 034-8628, Japan
Dr T. Uchide (e-mail uchide@vmas.kitasato-u.ac.jp) and Dr K. Saida (e-mail k.saida@aist.go.jp)
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Yuki FUJIMORI
;
Yuki FUJIMORI
*
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 35-1, Higashi 23-bancho, Towada, Aomori 034-8628, Japan
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Takushi SASAKI
;
Takushi SASAKI
*
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 35-1, Higashi 23-bancho, Towada, Aomori 034-8628, Japan
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Kyosuke TEMMA
;
Kyosuke TEMMA
*
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 35-1, Higashi 23-bancho, Towada, Aomori 034-8628, Japan
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Javier ADUR
;
Javier ADUR
†
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan‡
Microscopy Laboratory, Faculty of Bioengineering, National University of Entre Rios, Ruta 11, Km 101/2 (3101) Oro Verde, Entre Rios, Argentina
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Yoshinori MASUO
;
Yoshinori MASUO
†
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
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Takaharu KOZAKAI
;
Takaharu KOZAKAI
†
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
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Yun-Sik LEE
;
Yun-Sik LEE
†
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
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Kaname SAIDA
†
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
Dr T. Uchide (e-mail uchide@vmas.kitasato-u.ac.jp) and Dr K. Saida (e-mail k.saida@aist.go.jp)
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Clin Sci (Lond) (2002) 103 (s2002): 167S-170S.
Citation
Tsuyoshi UCHIDE, Yuki FUJIMORI, Takushi SASAKI, Kyosuke TEMMA, Javier ADUR, Yoshinori MASUO, Takaharu KOZAKAI, Yun-Sik LEE, Kaname SAIDA; Expression of endothelin-1 and vasoactive intestinal contractor genes in mouse organs during the perinatal period. Clin Sci (Lond) 1 September 2002; 103 (s2002): 167S–170S. doi: https://doi.org/10.1042/CS103S167S
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