Pericardial fluid accumulates the cardioprotective purine metabolites, as well as the endogenous vasoconstrictor agent endothelin-1 (ET-1). The aim of the present study was to characterize the pericardial concentrations of the purine metabolites adenosine, inosine and hypoxanthine before and after intrapericardial administration of ET-1 to the in situ heart. The closed pericardial sac of anaesthetized dogs (n = 9) was cannulated for ET-1 administration and for obtaining native pericardial fluid and control pericardial infusate samples (C1 and C2), as well as consecutive pericardial infusate samples (samples I, II and III) obtained 15min after intrapericardial administration of 150pmol/kg ET-1. In an additional five dogs, using the same protocol, ventricular epicardial and endocardial monophasic action potential recordings were performed to assess local ischaemic electrophysiological changes. Significant elevations of pericardial purine metabolite concentrations (measured by HPLC) were found in sample II compared with sample C2: adenosine, 4.5±1.7 compared with 0.5±0.1µM (P<0.05); inosine, 18.3±2.8 compared with 0.9±0.2µM (P<0.001); hypoxanthine, 38.1±8.0 compared with 13.4±2.6µM (P<0.01). Systemic blood pressure, left ventricular pressure and contractility, and systemic plasma levels of the purine metabolites remained unchanged during the ET-1 effect, while significant ECG ST elevations (STmax 0.68±0.01mV; P<0.001) were observed. In the five dogs analysed electrophysiologically, the left ventricular epicardial monophasic action potential duration and upstroke velocity decreased significantly at time point II compared with C2, while the endocardial monophasic action potential duration and upstroke velocity did not show ischaemia-related changes. The results suggest that intrapericardial administration of ET-1 induces subepicardial ischaemia, with parallel activation of coronary metabolic adaptive and cardiac self-protective mechanisms in the epimyocardial layer of the heart.
Skip Nav Destination
Article navigation
September 2002
-
Cover Image
Cover Image
Conference Article|
September 01 2002
Endothelin-1-induced elevations in purine metabolite concentrations — autoregulatory protection in the canine pericardium?
Endre ZIMA;
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Dr Endre Zima (e-mail [email protected]).
Search for other works by this author on:
Violetta KÉKESI;
Violetta KÉKESI
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Andrea NAGY;
Andrea NAGY
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
László LOSONCZI;
László LOSONCZI
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Ildikó TOMA;
Ildikó TOMA
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Pál SOÓS;
Pál SOÓS
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Erzsébet Barát;
Erzsébet Barát
†National Institute for TBC and Pulmonology, Pihenő út 1., H-1528 Budapest, Hungary
Search for other works by this author on:
Éva HUSZÁR;
Éva HUSZÁR
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Béla MERKELY;
Béla MERKELY
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Ferenc HORKAY;
Ferenc HORKAY
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Alexander Juhász-Nagy
Alexander Juhász-Nagy
*Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68., H-1122 Budapest, Hungary
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2002 The Biochemical Society and the Medical Research Society
2002
Clin Sci (Lond) (2002) 103 (s2002): 202S–205S.
Citation
Endre ZIMA, Violetta KÉKESI, Andrea NAGY, László LOSONCZI, Ildikó TOMA, Pál SOÓS, Erzsébet Barát, Éva HUSZÁR, Béla MERKELY, Ferenc HORKAY, Alexander Juhász-Nagy; Endothelin-1-induced elevations in purine metabolite concentrations — autoregulatory protection in the canine pericardium?. Clin Sci (Lond) 1 September 2002; 103 (s2002): 202S–205S. doi: https://doi.org/10.1042/CS103S202S
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |