Endothelin-1 (ET-1) is considered to be involved in the development and progression of heart failure. Therefore, we analysed the expression of endothelin-converting enzyme-1 (ECE-1), endothelin receptors A (ETA) and B (ETB) mRNAs by standard-calibrated, competitive reverse transcriptase-PCR using an internal-deleted in vitro-transcribed cRNA standard. ET-1 peptide levels were measured using isoform-specific rabbit antibodies against synthetic ET-1. mRNA and protein expression was determined in the right atrial myocardium of New York Heart Association class I patients and class IV patients undergoing aorto-coronary bypass surgery. ECE-1 mRNA was upregulated in failing atrial myocardium. Furthermore, ET-1 peptide levels were increased in failing atrial myocardium. Atrial ETA mRNA expression was not changed, while ETB mRNA was downregulated in the failing atrial myocardium. Our results support an upregulation of ET-1 synthesis by induction of ECE-1 in failing atrial myocardium. Pharmacological inhibition of augmented ECE-1 expression might provide a new therapeutic perspective in the treatment of heart failure.
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September 2002
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Conference Article|
September 01 2002
Increased expression of endothelin-converting enzyme-1 in failing human myocardium
Henning MORAWIETZ;
*Institute of Pathophysiology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Magdeburger Strasse 18, D-06097 Halle, Germany
Dr H. Morawietz (e-mail [email protected]).
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Winfried GOETTSCH;
Winfried GOETTSCH
*Institute of Pathophysiology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Magdeburger Strasse 18, D-06097 Halle, Germany
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Marten SZIBOR;
Marten SZIBOR
*Institute of Pathophysiology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Magdeburger Strasse 18, D-06097 Halle, Germany
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Matthias BARTON;
Matthias BARTON
†Department of Medicine and Medical Policlinic, University Hospital Zürich, Rämistrasse 100, CH-8091 Zürich, Switzerland
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Sidney SHAW;
Sidney SHAW
‡Department of Clinical Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland
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Kavous HAKIM;
Kavous HAKIM
§Clinic for Cardiothoracic Surgery, Martin Luther University Halle-Wittenberg, Ernst-Grube-Strasse 40, D-06097 Halle, Germany
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Hans-Reinhard ZERKOWSKI;
Hans-Reinhard ZERKOWSKI
‖Division of Cardiothoracic Surgery, University of Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland
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Juergen HOLTZ
Juergen HOLTZ
*Institute of Pathophysiology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Magdeburger Strasse 18, D-06097 Halle, Germany
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Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2002 The Biochemical Society and the Medical Research Society
2002
Clin Sci (Lond) (2002) 103 (s2002): 237S–240S.
Citation
Henning MORAWIETZ, Winfried GOETTSCH, Marten SZIBOR, Matthias BARTON, Sidney SHAW, Kavous HAKIM, Hans-Reinhard ZERKOWSKI, Juergen HOLTZ; Increased expression of endothelin-converting enzyme-1 in failing human myocardium. Clin Sci (Lond) 1 September 2002; 103 (s2002): 237S–240S. doi: https://doi.org/10.1042/CS103S237S
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