The purpose of the study was to assess whether increased pulmonary flow and subsequent development of pulmonary vascular remodelling could alter the expression of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) in the rat lung. Nine 42-day-old Wistar rats underwent abdominal aortocaval shunt to increase pulmonary blood flow for 12 weeks. The shunt resulted in significant medial hypertrophy of pulmonary artery without significant alterations in pulmonary or systemic blood pressure. Using competitive reverse transcription–PCR, significant increases in the preproET-1 mRNA expression and eNOS mRNA expression in the lungs of rats with abdominal aortocaval shunt were detected. Increased eNOS protein in the lung of shunt rats was also found by Western blot analysis. However, the plasma ET-1 concentration in the pulmonary artery (sham: 5±0.7pg/ml; shunt: 6±0.8pg/ml) or the lung ET-1 content (sham: 218±41ng/g protein; shunt: 224±40ng/g protein) was unchanged. There was an elevated immunohistochemical expression of eNOS, but not ET-1, in the pulmonary vascular endothelium in rats with the shunt. These results suggest that eNOS and ET-1 may be involved in remodelling prior to the development of pulmonary hypertension.
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September 2002
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Conference Article|
September 01 2002
Effects of increased pulmonary flow on the expression of endothelial nitric oxide synthase and endothelin-1 in the rat
Zen-Kong DAI;
Zen-Kong DAI
*Division of Cardiology and Pulmonology, Department of Pediatrics, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan
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Mian-Shin TAN;
Mian-Shin TAN
†School of Biology, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan
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Chee-Yin CHAI;
Chee-Yin CHAI
‡Department of Pathology, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan
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Ing-Jun CHEN;
Ing-Jun CHEN
§Department of Pharmacology, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan
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Arco Y. JENG;
Arco Y. JENG
‖Metabolic and Cardiovascular Diseases Research, Novartis Institute for Biomedical Research, 556 Morris Avenue, Summit, NJ 07901, U.S.A.
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Jiunn-Ren WU
*Division of Cardiology and Pulmonology, Department of Pediatrics, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan
Dr J.-R Wu (e-mail [email protected]).
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Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2002 The Biochemical Society and the Medical Research Society
2002
Clin Sci (Lond) (2002) 103 (s2002): 289S–293S.
Citation
Zen-Kong DAI, Mian-Shin TAN, Chee-Yin CHAI, Ing-Jun CHEN, Arco Y. JENG, Jiunn-Ren WU; Effects of increased pulmonary flow on the expression of endothelial nitric oxide synthase and endothelin-1 in the rat. Clin Sci (Lond) 1 September 2002; 103 (s2002): 289S–293S. doi: https://doi.org/10.1042/CS103S289S
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