Evidence has accumulated showing that vasoactive peptides, such as endothelin-1, adrenomedullin and urotensin-II, are expressed in various kinds of tumour cells. In the present study, the expression of endothelin-1 and endothelin receptors was studied in eight human tumour cell lines: T98G (glioblastoma), IMR-32 and NB69 (neuroblastoma), BeWo (choriocarcinoma), SW-13 (adrenocortical carcinoma), DLD-1 (colonic carcinoma), HeLa (cervical carcinoma) and VMRC-RCW (renal carcinoma). Reverse transcriptase–PCR showed expression of endothelin-1 mRNA in seven out of the eight cell lines, the exception being BeWo cells. ETA receptor mRNA was expressed in T98G, IMR-32 and NB69 cells, but weakly in the other cells. ETB receptor mRNA was expressed in IMR-32, NB69 and BeWo cells, but only weakly in T98G and HeLa cells. Immunoreactive endothelin was detected in the culture media of six out of the eight cell lines, but not in that of IMR-32 or BeWo cells. Treatment of T98G cells with an anti-endothelin-1 antibody or an anti-adrenomedullin antibody for 24h decreased cell numbers to approx. 84% and 90% of control respectively. Treatment with the ETA receptor antagonist BQ-610 (1µM) significantly decreased cell number to about 90% of control, whereas the ETB receptor antagonist BQ-788 had no significant effect. On the other hand, exogenously added endothelin-1, adrenomedullin or urotensin-II (0.1µM) had no significant effects on cell number. These results suggest that endothelin-1 acts as a paracrine or autocrine growth stimulator in tumours. The effect of endothelin-1 on tumour growth appears to be mediated by the ETA receptor.
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September 2002
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Conference Article|
September 01 2002
Three vasoactive peptides, endothelin-1, adrenomedullin and urotensin-II, in human tumour cell lines of different origin: expression and effects on proliferation
Kazuhiro TAKAHASHI;
*Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
Dr Kazuhiro Takahashi (e-mail [email protected]).
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Kazuhito TOTSUNE;
Kazuhito TOTSUNE
†Department of Medicine, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
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Tomomi KITAMURO;
Tomomi KITAMURO
*Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
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Masahiko SONE;
Masahiko SONE
*Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
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Osamu MURAKAMI;
Osamu MURAKAMI
†Department of Medicine, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
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Shigeki SHIBAHARA
Shigeki SHIBAHARA
*Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
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Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2000 The Biochemical Society and the Medical Research Society
2000
Clin Sci (Lond) (2002) 103 (s2002): 35S–38S.
Citation
Kazuhiro TAKAHASHI, Kazuhito TOTSUNE, Tomomi KITAMURO, Masahiko SONE, Osamu MURAKAMI, Shigeki SHIBAHARA; Three vasoactive peptides, endothelin-1, adrenomedullin and urotensin-II, in human tumour cell lines of different origin: expression and effects on proliferation. Clin Sci (Lond) 1 September 2002; 103 (s2002): 35S–38S. doi: https://doi.org/10.1042/CS103S035S
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