In this study, we investigated the production of endothelin 1 (ET-1) by a human microvascular endothelial cell line, HMEC-1, co-cultured with Plasmodium falciparum-parasitized red blood cells (pRBCs). The results indicate that hypoxia increased the basal level of ET-1 production by HMEC-1 cells after 24 or 48h of treatment. However, the co-incubation of HMEC-1 cells with pRBCs, but not with uninfected RBCs, induced a dose-dependent decrease of both constitutive and hypoxia-induced ET-1 production. The inhibition was not due to a decrease in cell viability, as lactate dehydrogenase release remained constant. These results indicate that pRBCs are able to interfere with both the constitutive and stimulated ET-1 release from the microvascular endothelium, thus inducing local modifications of the vascular tone and of the inflammatory response. This could be of relevance in the pathogenesis of the most severe forms of P. falciparum infections, such as cerebral malaria or malaria during pregnancy.
Skip Nav Destination
Article navigation
Conference Article|
September 01 2002
Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells
Nicoletta BASILICO;
*Institute of Microbiology, University of Milan, Via Pascal 36, 20133 Milan, Italy
Dr N. Basilico (e-mail [email protected]).
Search for other works by this author on:
Livianna SPECIALE;
Livianna SPECIALE
†Laboratory of Biology, Don C. Gnocchi Foundation, Istituto di Ricovero e Cura a Carattere Scientifico, Via Capecelatro, 66, I-20148, Milan, Italy
Search for other works by this author on:
Silvia PARAPINI;
Silvia PARAPINI
*Institute of Microbiology, University of Milan, Via Pascal 36, 20133 Milan, Italy
Search for other works by this author on:
Pasquale FERRANTE;
Pasquale FERRANTE
†Laboratory of Biology, Don C. Gnocchi Foundation, Istituto di Ricovero e Cura a Carattere Scientifico, Via Capecelatro, 66, I-20148, Milan, Italy
‡Department of Preclinical Sciences, University of Milan, Milan 20148, Italy
Search for other works by this author on:
Donatella TARAMELLI
Donatella TARAMELLI
*Institute of Microbiology, University of Milan, Via Pascal 36, 20133 Milan, Italy
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2002 The Biochemical Society and the Medical Research Society
2002
Clin Sci (Lond) (2002) 103 (s2002): 464S–466S.
Citation
Nicoletta BASILICO, Livianna SPECIALE, Silvia PARAPINI, Pasquale FERRANTE, Donatella TARAMELLI; Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells. Clin Sci (Lond) 1 September 2002; 103 (s2002): 464S–466S. doi: https://doi.org/10.1042/CS103S464S
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |