Blood pressure (BP) and heart rate (HR) in endothelin-3 (ET-3) null (-/-) knockout mice and ETA receptor (-/-) mice were measured using the servo null pressure measuring technique under halothane anaesthesia. In infant ET-3 (-/-) mice (2–3 weeks old), mean BP and HR were 55±2mmHg and 436±30beats/min respectively. These values were not different from those in age-matched wild-type mice (53±3mmHg and 430±18beats/min respectively). Baroreflex sensitivity, which was calculated as the slope of the relationship between systolic BP and RR interval on an ECG, was also similar in ET-3 (-/-) mice (0.84±0.20ms/mmHg) and wild-type mice (1.07±0.38ms/mmHg). ETA receptor (-/-) mice were obtained by caesarean section on the expected day of delivery and tracheotomized, so that they would live for more than 24h. Mean BP and HR in ETA receptor (-/-) mice were 15±1mmHg and 333±6beats/min respectively. These values were not different from those in age-matched, similarly treated wild-type mice (16±3mmHg and 308±10beats/min respectively). Baroreflex sensitivity in the newborn ETA receptor (-/-) mice (0.45±0.15ms/mmHg) and wild-type mice (0.31±0.06ms/mmHg) were very low compared with the values in infant wild-type mice, but not different between the mutant mice and their littermates. Moreover, HR in awake ETA receptor (-/-) mice (396±13beats/min) was not different from that in wild-type mice (409±13beats/min). These results show that the ETA receptor and ET-3 are not involved in cardiovascular regulation, at least during the very early life of the mice. A possible involvement of the ETA receptor in BP regulation, if any, seems to occur at later times and/or in some pathological settings.
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September 2002
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September 01 2002
Blood pressure of endothelin-3 null (-/-) knockout mice and endothelin A receptor null (-/-) knockout mice under anaesthesia
Tomoyuki KUWAKI;
*Department of Molecular and Integrative Physiology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
†Department of Autonomic Physiology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
Professor Tomoyuki Kuwaki, at Department of Molecular and Integrative Physiology (e-mail [email protected]).
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Toru ISHII;
Toru ISHII
†Department of Autonomic Physiology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
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Kihwan JU;
Kihwan JU
‡Department of Biomedical Engineering, School of Engineering, The City University of New York, NY 10031, U.S.A.
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Masashi YANAGISAWA;
Masashi YANAGISAWA
§Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75235, U.S.A.
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Yasuichiro FUKUDA
Yasuichiro FUKUDA
†Department of Autonomic Physiology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
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Publisher: Portland Press Ltd
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2002 The Biochemical Society and the Medical Research Society
2002
Clin Sci (Lond) (2002) 103 (s2002): 48S–52S.
Citation
Tomoyuki KUWAKI, Toru ISHII, Kihwan JU, Masashi YANAGISAWA, Yasuichiro FUKUDA; Blood pressure of endothelin-3 null (-/-) knockout mice and endothelin A receptor null (-/-) knockout mice under anaesthesia. Clin Sci (Lond) 1 September 2002; 103 (s2002): 48S–52S. doi: https://doi.org/10.1042/CS103S048S
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