The aim of the present study was to quantify β2-adrenoceptor protein content in adipose tissue during fasting, and to study the relationships between β2-adrenoceptor protein and mRNA levels and changes in metabolites related to lipolysis. Groups of male subjects with a body mass index of <25kg/m2 or >30kg/m2 fasted for 60h. Abdominal subcutaneous fat biopsies were analysed for receptor mRNA levels by reverse transcription–PCR–HPLC. The β2-adrenoceptor protein concentration was measured by Western blotting using fluorescence laser scanning for detection. The β2-adrenoceptor protein concentration per cell (on a DNA basis) was higher in obese subjects (P<0.03). There were highly significant relationships between β2-adrenoceptor protein concentration and both body mass index and waist/hip ratio (P<0.001 for both). Furthermore, there was an inverse relationship between the receptor protein concentration and the serum β-hydroxybutyrate level during fasting (P<0.005). β2-Adrenoceptor protein levels decreased in both groups during fasting, to a similar degree. Basal β2-adrenoceptor mRNA levels were similar in the two groups, but there was a smaller increase in the obese group during fasting (P<0.03). The increased β2-adrenoceptor protein level in obese subjects is likely to be related to the greater plasma membrane area of their adipocytes. The decrease during fasting may be due to increased binding of noradrenaline and subsequent internalization and degradation of the receptor. Elevated levels of less responsive β2-adrenoceptor protein in obese subjects may contribute to the development of obesity.
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January 09 2003
Elevated β2-adrenoceptor protein concentration in adipose tissue from obese subjects is closely related to the body mass index and waist/hip ratio
Mads RASMUSSEN;
1Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
Correspondence: Dr Mads Rasmussen (e-mail [email protected]).
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Thomas ALMDAL;
Thomas ALMDAL
1Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Palle BRATHOLM;
Palle BRATHOLM
1Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Niels Juel CHRISTENSEN
Niels Juel CHRISTENSEN
1Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Publisher: Portland Press Ltd
Received:
August 07 2002
Revision Received:
October 14 2002
Accepted:
November 13 2002
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2003
2003
Clin Sci (Lond) (2003) 104 (2): 93–102.
Article history
Received:
August 07 2002
Revision Received:
October 14 2002
Accepted:
November 13 2002
Citation
Mads RASMUSSEN, Thomas ALMDAL, Palle BRATHOLM, Niels Juel CHRISTENSEN; Elevated β2-adrenoceptor protein concentration in adipose tissue from obese subjects is closely related to the body mass index and waist/hip ratio. Clin Sci (Lond) 1 February 2003; 104 (2): 93–102. doi: https://doi.org/10.1042/cs1040093
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