MOCP (maternal obstructive cholestasis during pregnancy) induces a reversible impairment in bile formation in young rats born to these mothers. The aim of the present study was to gain information on the effects of MOCP on the maturation of pathways involved in protein secretion into bile in young (4-week-old) rats. The amount of hepatic α-tubulin and the structure of the microtubular network were apparently not affected by MOCP. HRP (horseradish peroxidase) was used as a model protein, and its secretion into bile after administration through the jugular vein was measured. In adult (8-week-old) rats, two peaks of HRP output into bile were observed following administration: an early peak presumably due to paracellular transfer, and a late peak presumably due to transcytosis. In young rats (4 weeks old), the early peak was similar to that of adult animals, and was not affected by MOCP. However, the late peak was markedly smaller in young control rats, and was further reduced by MOCP. Brefeldin A decreased, whereas taurocholate did not change, the early peak, whereas both affected the transcytotic transport of HRP. Brefeldin A delayed HRP secretion (similarly in control and MOCP groups), without affecting cumulative output, whereas taurocholate accelerated the transcytotic transport of HRP in the control group, but not in the MOCP group. These results suggest that MOCP affects the maturation of hepatocyte mechanisms involved in the transcytotic secretion of HRP into bile.
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Research Article|
September 01 2003
Effect of maternal obstructive cholestasis during pregnancy on the biliary transport of horseradish peroxidase in the rat offspring
Maria J. MONTE;
Maria J. MONTE
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
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Gloria R. VILLANUEVA;
Gloria R. VILLANUEVA
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
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Rocio I. R. MACIAS;
Rocio I. R. MACIAS
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
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David J. VAZQUEZ;
David J. VAZQUEZ
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
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Marta TOLEDO;
Marta TOLEDO
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
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Mercedes DOMINGUEZ;
Mercedes DOMINGUEZ
†Servicio de Inmunología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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Jose J. G. MARIN
*Departamento de Fisiologia y Farmacologia, Campus Miguel Unamuno, EID-S09, 37007-Salamanca, Spain
Correspondence: Dr Jose J. G. Marin (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
March 26 2003
Revision Received:
May 12 2003
Accepted:
May 27 2003
Accepted Manuscript online:
May 27 2003
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2003 The Biochemical Society
2003
Clin Sci (Lond) (2003) 105 (3): 347–353.
Article history
Received:
March 26 2003
Revision Received:
May 12 2003
Accepted:
May 27 2003
Accepted Manuscript online:
May 27 2003
Citation
Maria J. MONTE, Gloria R. VILLANUEVA, Rocio I. R. MACIAS, David J. VAZQUEZ, Marta TOLEDO, Mercedes DOMINGUEZ, Jose J. G. MARIN; Effect of maternal obstructive cholestasis during pregnancy on the biliary transport of horseradish peroxidase in the rat offspring. Clin Sci (Lond) 1 September 2003; 105 (3): 347–353. doi: https://doi.org/10.1042/CS20030114
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