The bioactive and potent androgen 5α-dihydrotestosterone (DHT) has been postulated to be involved in the development of branching morphogenesis in the human fetal lung, but its expression has not been examined. We therefore examined the expression of the androgen receptor (AR) and 5α-reductases (type 1 and type 2), which catalyse the conversion of testosterone into DHT, in the human fetal lung using immunohistochemistry and reverse transcription–PCR (RT-PCR). Immunoreactive AR was detected predominantly in the nuclei of epithelial cells of the budding component of the early gestational fetal lung. 5α-Reductase type 1 immunoreactivity was detected in the cytoplasm of epithelial cells, whereas immunoreactivity for 5α-reductase type 2 was not detected in the samples of human fetal lung examined. RT-PCR also confirmed the presence of AR and 5α-reductase in all fetal lung and epithelial cell lines. The results of our present study suggest that DHT may play an important role in epithelial cells, which might include precursor cells, in which both AR and 5α-reductases are expressed during branching morphogenesis of the human fetal lung.
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December 2003
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Research Article|
December 01 2003
Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung: a possible correlation with branching morphogenesis
Y. KIMURA;
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
†Department of Respiratory Oncology/Molecular Medicine, Tohoku University, Sendai, Japan
Correspondence: Dr Yuichiro Kimura, Department of Pathology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan (e-mail kimuyu@patholo2.med.tohoku.ac.jp).
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T. SUZUKI;
T. SUZUKI
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
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C. KANEKO;
C. KANEKO
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
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A. D. DARNEL;
A. D. DARNEL
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
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J. AKAHIRA;
J. AKAHIRA
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
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M. EBINA;
M. EBINA
†Department of Respiratory Oncology/Molecular Medicine, Tohoku University, Sendai, Japan
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T. NUKIWA;
T. NUKIWA
†Department of Respiratory Oncology/Molecular Medicine, Tohoku University, Sendai, Japan
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H. SASANO
H. SASANO
*Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
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Clin Sci (Lond) (2003) 105 (6): 709–713.
Article history
Received:
July 14 2003
Accepted:
August 01 2003
Accepted Manuscript online:
August 01 2003
Citation
Y. KIMURA, T. SUZUKI, C. KANEKO, A. D. DARNEL, J. AKAHIRA, M. EBINA, T. NUKIWA, H. SASANO; Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung: a possible correlation with branching morphogenesis. Clin Sci (Lond) 1 December 2003; 105 (6): 709–713. doi: https://doi.org/10.1042/CS20030236
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