The quantity of deuterated glucose customarily given in labelled IVGTTs (intravenous glucose tolerance tests) changes the isotopic composition of the subject's body water enough to be detected by mass spectrometric techniques. Glucose undergoing direct glycogenesis does not contribute label to the body water pool, and isotope incorporated into it must have come from glucose that has either been oxidized or undergone indirect glycogenesis. By subtracting the amount of label found in body water from the total amount of glucose utilized, as calculated from the minimal model of glucose disappearance, it should be possible to study the partitioning of the dose given between direct glycogenesis in skeletal muscle and other metabolic pathways. To establish these principles, we used isotope ratio MS to determine body water composition in groups of healthy (n=7; mean weight, 76 kg; fasting plasma glucose and insulin, 5.1 mmol and 40 pmol respectively) and Type II diabetic (n=5; mean weight, 84 kg; fasting plasma glucose and insulin, 6.2 mmol and 75 pmol respectively) subjects undergoing IVGTTs. It was found that, for healthy subjects, 31% of the dose given was utilized in direct glycogenesis and this was decreased to 15% in diabetes. Defects in muscle glycogen synthesis in diabetes of the same order are well known from magnetic resonance studies. We conclude that measurement of label incorporation into body water is potentially useful for investigation of the metabolism of a glucose load in vivo during an IVGTT.
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June 01 2004
Glycogenesis and glucose oxidation during an intravenous glucose tolerance test in man
Leslie J. C. BLUCK;
1MRC Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
Correspondence: Dr Leslie Bluck (e-mail [email protected]).
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Allan T. CLAPPERTON;
Allan T. CLAPPERTON
1MRC Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
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Cheryl V. KIDNEY;
Cheryl V. KIDNEY
1MRC Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
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W. Andy COWARD
W. Andy COWARD
1MRC Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
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Publisher: Portland Press Ltd
Received:
October 28 2003
Revision Received:
January 30 2004
Accepted:
February 04 2004
Accepted Manuscript online:
February 04 2004
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2004 The Biochemical Society
2004
Clin Sci (Lond) (2004) 106 (6): 645–652.
Article history
Received:
October 28 2003
Revision Received:
January 30 2004
Accepted:
February 04 2004
Accepted Manuscript online:
February 04 2004
Citation
Leslie J. C. BLUCK, Allan T. CLAPPERTON, Cheryl V. KIDNEY, W. Andy COWARD; Glycogenesis and glucose oxidation during an intravenous glucose tolerance test in man. Clin Sci (Lond) 1 June 2004; 106 (6): 645–652. doi: https://doi.org/10.1042/CS20030353
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