The aim of the present study was to examine gene expression and protein concentrations of β1- and β2-adrenergic receptors in subcutaneous adipose tissue in obese subjects in response to weight loss. Eighteen obese subjects were studied during diet-induced weight loss. β-Adrenergic receptor mRNA levels were quantified by reverse transcription-PCR–HPLC. β-Adrenergic receptor protein concentrations were measured by Western blotting using fluorescence laser scanning for detection. Subjects lost 12.8±0.8 kg (mean±S.E.M.) during diet treatment. There was a 34% decrease in the β1-adrenergic receptor mRNA level (0.92±0.09 compared with 0.61±0.06 amol/μg of DNA; P<0.002). β2-Adrenergic receptor mRNA did not decrease significantly. β2-Adrenergic receptor protein concentration decreased 37% (25.5±7.1 compared with 16.0±5.6 arbitrary units/ng of DNA; P=0.008), whereas β1-adrenergic receptor protein concentration did not decrease significantly. The degree of weight loss was correlated with the concentration of β1-adrenergic receptor protein (r=0.65, P<0.003) and changes in receptor protein concentration (r=0.50, P=0.035) during the very-low-calorie diet. In conclusion, the present study demonstrates a relationship between β1-adrenergic receptor protein concentration in adipose tissue and the degree of weight loss. This relationship is not directly related to energy expenditure and deserves further investigation.
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April 2005
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Research Article|
March 22 2005
Change in β1-adrenergic receptor protein concentration in adipose tissue correlates with diet-induced weight loss
Mads RASMUSSEN;
*Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
Correspondence: Dr Mads Rasmussen (email [email protected]).
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Anita BELZA;
Anita BELZA
†Department of Human Nutrition, The Royal Veterinary and Agricultural University, 2000 Frederiksberg, Denmark
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Thomas ALMDAL;
Thomas ALMDAL
*Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Søren TOUBRO;
Søren TOUBRO
†Department of Human Nutrition, The Royal Veterinary and Agricultural University, 2000 Frederiksberg, Denmark
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Palle BRATHOLM;
Palle BRATHOLM
*Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Arne ASTRUP;
Arne ASTRUP
†Department of Human Nutrition, The Royal Veterinary and Agricultural University, 2000 Frederiksberg, Denmark
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Niels J. CHRISTENSEN
Niels J. CHRISTENSEN
*Department of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark
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Publisher: Portland Press Ltd
Received:
August 10 2004
Revision Received:
November 04 2004
Accepted:
December 01 2004
Accepted Manuscript online:
December 01 2004
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 108 (4): 323–329.
Article history
Received:
August 10 2004
Revision Received:
November 04 2004
Accepted:
December 01 2004
Accepted Manuscript online:
December 01 2004
Citation
Mads RASMUSSEN, Anita BELZA, Thomas ALMDAL, Søren TOUBRO, Palle BRATHOLM, Arne ASTRUP, Niels J. CHRISTENSEN; Change in β1-adrenergic receptor protein concentration in adipose tissue correlates with diet-induced weight loss. Clin Sci (Lond) 1 April 2005; 108 (4): 323–329. doi: https://doi.org/10.1042/CS20040238
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