TACE [TNF-α (tumour necrosis factor-α)-converting enzyme] plays an essential role in the shedding of TNF-α, which could affect the outcome of AMI (acute myocardial infarction). To investigate the clinical significance of the TACE–TNF-α system in AMI, we examined TACE-mediated TNF-α synthesis in PBMCs (peripheral blood mononuclear cells), which are a possible source of TNF-α in AMI. Forty-one patients with AMI and 15 healthy subjects (HS) were enrolled in the present study. PBMCs were isolated from peripheral blood on day 1 and 14 after the onset of AMI. TACE and TNF-α mRNA levels and intracellular median fluorescence intensity were measured by real-time RT (reverse transcriptase)–PCR and flow cytometry respectively. TACE-mediated TNF-α production was evaluated in cultured PBMCs with PMA, which is known to activate TACE. Spontaneous TACE and TNF-α levels were higher in AMI patients than in HS (P<0.001). TACE and TNF-α levels in PMA-stimulated PMBCs were markedly increased in AMI patients compared with HS (P<0.001). There was a positive correlation between TACE and TNF-α levels in AMI. Although spontaneous and stimulated levels of TACE and TNF-α decreased 14 days after the onset of AMI, levels in AMI patients were higher than in HS. In AMI patients with in-hospital complications (n=15; pump failure in ten, recurrent myocardial infarction in one, malignant ventricular arrhythmia in three and cardiac death in one), spontaneous and stimulated levels of TACE and TNF-α were higher than in patients without complications (P<0.01). These levels were higher in AMI patients with in-hospital complications 14 days after onset. These results demonstrate that TACE-mediated TNF-α maturation in PBMCs may play an important role in poor outcomes from AMI, suggesting that TACE may be a potential target for the inhibition of cellular TNF-α production in AMI.
Skip Nav Destination
Article navigation
April 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
March 22 2005
Activated tumour necrosis factor-α shedding process is associated with in-hospital complication in patients with acute myocardial infarction
Yudai SHIMODA;
Yudai SHIMODA
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Uchimaru 19-1, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Mamoru SATOH;
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Uchimaru 19-1, Morioka 020-8505, Iwate, Japan
Correspondence: Dr Mamoru Satoh (email [email protected]).
Search for other works by this author on:
Motoyuki NAKAMURA;
Motoyuki NAKAMURA
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Uchimaru 19-1, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Tomonari AKATSU;
Tomonari AKATSU
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Uchimaru 19-1, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Katsuhiko HIRAMORI
Katsuhiko HIRAMORI
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Uchimaru 19-1, Morioka 020-8505, Iwate, Japan
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 05 2004
Revision Received:
November 29 2004
Accepted:
December 17 2004
Accepted Manuscript online:
December 17 2004
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 108 (4): 339–347.
Article history
Received:
August 05 2004
Revision Received:
November 29 2004
Accepted:
December 17 2004
Accepted Manuscript online:
December 17 2004
Citation
Yudai SHIMODA, Mamoru SATOH, Motoyuki NAKAMURA, Tomonari AKATSU, Katsuhiko HIRAMORI; Activated tumour necrosis factor-α shedding process is associated with in-hospital complication in patients with acute myocardial infarction. Clin Sci (Lond) 1 April 2005; 108 (4): 339–347. doi: https://doi.org/10.1042/CS20040229
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |