In a cross-sectional study, oxidative stress in high vascular disease risk groups, ESRD (end-stage renal disease) and Type I diabetes, was assessed by measuring plasma protein carbonyls and comparing antioxidant capacity of HDL (high-density lipoprotein) as pertaining to PON1 (paraoxonase 1) activity and in vitro removal of LPO (lipid peroxides). ESRD subjects on haemodialysis (n=22), Type I diabetes subjects (n=20) without vascular complications and healthy subjects (n=23) were compared. Plasma protein carbonyls were higher in ESRD patients [0.16 (0.050) nmol/mg of protein; P=0.001; value is mean (SD)] relative to subjects with Type I diabetes [0.099 (0.014) nmol/mg of protein] and healthy subjects [0.093 (0.014) nmol/mg of protein]. Plasma PON1 activity, with and without correction for HDL-cholesterol, was lower in diabetes but did not differ in ESRD compared with healthy subjects. Plasma PON1 activity, without correction for HDL, did not differ between the three groups. In ESRD, plasma PON1 activity and plasma protein carbonyl concentrations were inversely related (r=−0.50, P<0.05). In an in vitro assay, LPO removal by HDL in ESRD subjects was greater than HDL from healthy subjects (P<0.01), whereas HDL from patients with Type I diabetes was less effective (P<0.01). Efficacy of LPO removal was unrelated to plasma PON1 activity, in vitro glycation or mild oxidation, but was impaired by marked oxidation and glycoxidation. Protein carbonyl levels are increased in ESRD but not in complication-free Type I diabetes. HDL antioxidant function is increased in ESRD, perhaps a compensatory response to increased oxidative stress, but is lower in Type I diabetes. HDL dysfunction is related to glycoxidation rather than glycation or PON1 activity.
Skip Nav Destination
Article navigation
June 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
May 24 2005
Oxidative stress and high-density lipoprotein function in Type I diabetes and end-stage renal disease
George KALOGERAKIS;
George KALOGERAKIS
1
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Arthur M. BAKER;
Arthur M. BAKER
1
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Steve CHRISTOV;
Steve CHRISTOV
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Kevin G. ROWLEY;
Kevin G. ROWLEY
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Karen DWYER;
Karen DWYER
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Christine WINTERBOURN;
Christine WINTERBOURN
†Christchurch School of Medicine and Health Sciences, Department of Pathology, Free Radical Research Group, Christchurch Hospital, Christchurch, New Zealand
Search for other works by this author on:
James D. BEST;
James D. BEST
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Search for other works by this author on:
Alicia J. JENKINS
*Department of Medicine, The University of Melbourne, St. Vincent's Hospital, Fitzroy, 3065, Melbourne, Australia
Correspondence: Dr Alicia Jenkins (email [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 27 2004
Revision Received:
December 15 2004
Accepted:
January 06 2005
Accepted Manuscript online:
January 06 2005
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 108 (6): 497–506.
Article history
Received:
October 27 2004
Revision Received:
December 15 2004
Accepted:
January 06 2005
Accepted Manuscript online:
January 06 2005
Citation
George KALOGERAKIS, Arthur M. BAKER, Steve CHRISTOV, Kevin G. ROWLEY, Karen DWYER, Christine WINTERBOURN, James D. BEST, Alicia J. JENKINS; Oxidative stress and high-density lipoprotein function in Type I diabetes and end-stage renal disease. Clin Sci (Lond) 1 June 2005; 108 (6): 497–506. doi: https://doi.org/10.1042/CS20040312
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |