Hypothermia is an important preservation method for tissues and solid organs. The aim of the present study was to assess in rat cremaster muscle the effect of hypothermia, without or with pre-ischaemic HTK (histidine-tryptophan-ketoglutarate–Bretschneider solution) perfusion, on microvascular consequences of 4 or 6 h ischaemia and 2 h of reperfusion. Intravital microscopy was applied to examine capillary perfusion and leucocyte–endothelium interactions. The cremaster muscle was subjected to 4 or 6 h of cold (4 °C) or warm (33–34 °C) ischaemia and 2 h of reperfusion. Measurements were performed at baseline, prior to HTK perfusion and ischaemia, and at 0, 1 and 2 h after blood flow restoration. Hypothermia completely prevented the 50% reduction in capillary perfusion that was observed previously at start of reperfusion after 4 h warm ischaemia. After 6 h of warm ischaemia, perfusion resumed in only 45% of capillaries and remained at this low level during reperfusion. In contrast, only a slight decrease (<10%) in capillary perfusion was observed after 6 h of cold ischaemia. Pre-ischaemic HTK perfusion had no beneficial effect on tissue perfusion. Both hypothermia and HTK attenuated the significant increase in venular leucocyte–vessel wall interactions, which was observed after 4 h of warm ischaemia in a previous study. Combined application of both interventions had no additional effects. After 6 h of warm ischaemia, no increase in leucocyte–vessel wall interactions was observed, possibly due to venular flow reduction. In conclusion, hypothermia preserves capillary perfusion and prevents an increase in leucocyte–vessel wall interactions during reperfusion after muscle tissue ischaemia. Preischaemic perfusion of the vasculature with HTK does not improve the effects of cold storage on tissue perfusion, but attenuates the inflammatory response independently of temperature effect.
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July 2005
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Research Article|
June 23 2005
Effect of hypothermia and HTK on the microcirculation in the rat cremaster muscle after ischaemia
Jacqueline BASTIAANSE;
*Department of Plastic, Reconstructive and Hand Surgery, Utrecht University Medical Center, 3508 GA Utrecht, The Netherlands
†Department of Plastic, Reconstructive and Hand Surgery, University Hospital Maastricht, 6202 AZ Maastricht, The Netherlands
Correspondence: Dr Jacqueline Bastiaanse, Department of Plastic and Reconstructive Surgery, University Hospital Maastricht, 6202 AZ Maastricht, The Netherlands (email [email protected]).
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Dick W. SLAAF;
Dick W. SLAAF
‡Department of Biophysics, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
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Mirjam G. A. oude EGBRINK;
Mirjam G. A. oude EGBRINK
§Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
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Gary L. ANDERSON;
Gary L. ANDERSON
∥Department of Physiology and Biophysics, University of Louisville, Louisville, KY 40292, U.S.A.
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Hans VINK;
Hans VINK
¶Department of Medical Physics, Academic Medical Center, 1100 DD Amsterdam, The Netherlands
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Brigitte E. P. A. van der HEIJDEN;
Brigitte E. P. A. van der HEIJDEN
*Department of Plastic, Reconstructive and Hand Surgery, Utrecht University Medical Center, 3508 GA Utrecht, The Netherlands
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Moshe KON
Moshe KON
*Department of Plastic, Reconstructive and Hand Surgery, Utrecht University Medical Center, 3508 GA Utrecht, The Netherlands
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Publisher: Portland Press Ltd
Received:
June 02 2004
Revision Received:
December 01 2004
Accepted:
December 21 2004
Accepted Manuscript online:
December 21 2004
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 109 (1): 117–123.
Article history
Received:
June 02 2004
Revision Received:
December 01 2004
Accepted:
December 21 2004
Accepted Manuscript online:
December 21 2004
Citation
Jacqueline BASTIAANSE, Dick W. SLAAF, Mirjam G. A. oude EGBRINK, Gary L. ANDERSON, Hans VINK, Brigitte E. P. A. van der HEIJDEN, Moshe KON; Effect of hypothermia and HTK on the microcirculation in the rat cremaster muscle after ischaemia. Clin Sci (Lond) 1 July 2005; 109 (1): 117–123. doi: https://doi.org/10.1042/CS20040154
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