In the present study, we have developed and demonstrated a coronary artery imaging protocol in rats using transthoracic high-frequency CDE (colour Doppler echocardiography) to investigate the potential direct effects of exercise on CFVR (coronary flow velocity reserve). SHR (spontaneously hypertensive rats) performed voluntary exercise for 6 weeks. Rats were then submitted to ultrasonographic examination and CFVR measurements. The LAD (left anterior descending coronary artery) was visualized using transthoracic CDE in a modified parasternal long-axis view. Doppler measurement was made in mid-LAD during baseline and adenosine-induced hyperaemic condition. Gene and protein expression in cardiac tissue were studied using real-time PCR and immunohistochemistry. Adenosine infusion significantly (P<0.001, as determined by ANOVA) decreased HR, without affecting blood pressure in anaesthetized SHR. A significantly greater adenosine dose-dependent response was seen in exercised rats compared with controls (P=0.02, as determined by ANOVA). The baseline flow velocity in mid-LAD was 0.33±0.06 and 0.41±0.14 m/s in the exercised and control animals respectively (P value was not significant). The maximum adenosine-induced response was reached at a dose of 140 μg·kg−1 of body weight·min−1, and CFVR averaged at 2.6±0.53 and 1.5±0.24 in exercised and control animals respectively (P<0.01). Gene expression of CuZnSOD was up-regulated by 21% in exercised animals compared with controls (1.1±0.16 compared with 0.89±0.09; P<0.01), whereas eNOS expression was unchanged. In conclusion, CFVR in rats can be non-invasively assessed using CDE with high feasibility. Physical exercise is associated with improved CFVR and antioxidative capacity in SHR.
Skip Nav Destination
Article navigation
September 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
August 24 2005
Voluntary physical exercise and coronary flow velocity reserve: a transthoracic colour Doppler echocardiography study in spontaneously hypertensive rats
Ulrika Hägg;
Ulrika Hägg
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
Search for other works by this author on:
Julia Grönros;
Julia Grönros
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
Search for other works by this author on:
Johannes Wikström;
Johannes Wikström
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
Search for other works by this author on:
Ingibjörg H. Jonsdottir;
Ingibjörg H. Jonsdottir
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
†Institute of Stress Medicine, Göteborg, Sweden
Search for other works by this author on:
Göran Bergström;
Göran Bergström
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
‡Department of Clinical Physiology, Sahlgrenska University Hospital, Göteborg, Sweden
Search for other works by this author on:
Li-ming Gan
*Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
‡Department of Clinical Physiology, Sahlgrenska University Hospital, Göteborg, Sweden
Correspondence: Dr Li-ming Gan, Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden (email [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
February 04 2005
Revision Received:
April 29 2005
Accepted:
May 26 2005
Accepted Manuscript online:
May 26 2005
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 109 (3): 325–334.
Article history
Received:
February 04 2005
Revision Received:
April 29 2005
Accepted:
May 26 2005
Accepted Manuscript online:
May 26 2005
Citation
Ulrika Hägg, Julia Grönros, Johannes Wikström, Ingibjörg H. Jonsdottir, Göran Bergström, Li-ming Gan; Voluntary physical exercise and coronary flow velocity reserve: a transthoracic colour Doppler echocardiography study in spontaneously hypertensive rats. Clin Sci (Lond) 1 September 2005; 109 (3): 325–334. doi: https://doi.org/10.1042/CS20050052
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |