In the present study, we have investigated the use of 1-[13C]glucose and GC/combustion/isotope-ratio MS as an alternative to 6,6-[2H2]glucose and GC/MS in the determination of parameters of glucose metabolism using the IVGTT (intravenous glucose tolerance test) interpreted by labelled (hot) minimal models. The study has been done in four populations, normoglycaemics (subdivided into lean and obese individuals), subjects with impaired glucose tolerance and those with diabetes mellitus. Although the use of carbon label may in some circumstances be compromised by substrate recycling, our hypothesis was that this would not be an issue under the condition of suppression of hepatic glucose production during the short timescale of an IVGTT. In all four groups, we found that the methodology employing the carbon label gave equivalent results to those obtained using the conventional deuterated material, but the sensitivity of the measurement technique in the new approach was sufficient to allow an approx. 15-fold reduction in the quantity of isotope administered. In addition to the clear cost advantages, this represents a significant scientific advance in that true tracer status is more nearly attained in these measurements with near-physiological tracee loads.
Skip Nav Destination
Article navigation
December 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
November 23 2005
13C- and 2H-labelled glucose compared for minimal model estimates of glucose metabolism in man
Leslie J. C. Bluck;
1Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
Correspondence: Dr Leslie J. C. Bluck (email [email protected]).
Search for other works by this author on:
Allan T. Clapperton;
Allan T. Clapperton
1Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
Search for other works by this author on:
W. Andy Coward
W. Andy Coward
1Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Peterhouse Park, Fulbourn Road, Cambridge CB1 9NL, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
May 12 2005
Revision Received:
August 03 2005
Accepted:
August 10 2005
Accepted Manuscript online:
August 10 2005
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 109 (6): 513–521.
Article history
Received:
May 12 2005
Revision Received:
August 03 2005
Accepted:
August 10 2005
Accepted Manuscript online:
August 10 2005
Citation
Leslie J. C. Bluck, Allan T. Clapperton, W. Andy Coward; 13C- and 2H-labelled glucose compared for minimal model estimates of glucose metabolism in man. Clin Sci (Lond) 1 December 2005; 109 (6): 513–521. doi: https://doi.org/10.1042/CS20050155
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |