In the present study, we investigated the vasodilator properties of A-type, B-type and C-type natriuretic peptides (ANP, BNP and CNP respectively) and the NO (nitric oxide) donor sin-1 (3-morpholino-sydnonimine) in human by-pass grafts. In contrast with previous studies, the same vessel was used to demonstrate a direct link between cGMP production and functional relaxation. Remnants of the IMA (internal mammary artery) and SV (saphenous vein) were obtained from 82 patients undergoing coronary artery by-pass grafting. The responses to cumulative concentrations of ANP, BNP, CNP and sin-1 in vessel rings pre-contracted with a thromboxane A2 agonist (U46619) were measured in an organ bath. Additionally, intracellular cGMP production after single submaximal dose application of these drugs to vessel rings was determined by a RIA. ANP (P=0.001) and sin-1 (P<0.001) caused significant concentration-dependent relaxation of the IMA. In the SV, only sin-1 (P<0.001) induced marked concentration-dependent relaxation. At a single submaximal concentration, significant relaxation as well as intracellular cGMP production were found in response to ANP, BNP and sin-1 in the IMA. In contrast, in the SV, only sin-1 significantly induced cGMP production and relaxation. There was a moderate, but significant, correlation between intracellular cGMP net production and net relaxation in the IMA. In conclusion, ANP, as the most powerful relaxant of all the natriuretic peptides tested on the IMA, may be a possible alternative vasorelaxant to overcome peri-operative vasospasm in this artery. In contrast with sin-1, ANP and BNP were not effective vasorelaxants of the SV. Net relaxation in response to natriuretic peptides correlated with cGMP net concentrations in the IMA.
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Research Article|
August 15 2006
Direct comparison of relaxation and cGMP production in human coronary by-pass grafts in response to stimulation with natriuretic peptides and a nitric oxide donor
Angelika Hammerer-Lercher;
*Division of Clinical Biochemistry, Innsbruck Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria
Correspondence: Dr Angelika Hammerer-Lercher (email [email protected]).
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Johann Fersterer;
Johann Fersterer
*Division of Clinical Biochemistry, Innsbruck Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Sigrid Holzmann;
Sigrid Holzmann
†Department of Pharmacology and Toxicology, University of Graz, A-8010 Graz, Austria
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Johannes Bonatti;
Johannes Bonatti
‡Department of Cardiac Surgery, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Elfriede Ruttmann;
Elfriede Ruttmann
‡Department of Cardiac Surgery, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Daniel Hoefer;
Daniel Hoefer
‡Department of Cardiac Surgery, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Johannes Mair;
Johannes Mair
§Department of Internal Medicine, Division of Cardiology, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Bernd Puschendorf
Bernd Puschendorf
*Division of Clinical Biochemistry, Innsbruck Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria
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Publisher: Portland Press Ltd
Received:
February 09 2006
Revision Received:
April 26 2006
Accepted:
May 18 2006
Accepted Manuscript online:
May 18 2006
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2006
Clin Sci (Lond) (2006) 111 (3): 225–231.
Article history
Received:
February 09 2006
Revision Received:
April 26 2006
Accepted:
May 18 2006
Accepted Manuscript online:
May 18 2006
Citation
Angelika Hammerer-Lercher, Johann Fersterer, Sigrid Holzmann, Johannes Bonatti, Elfriede Ruttmann, Daniel Hoefer, Johannes Mair, Bernd Puschendorf; Direct comparison of relaxation and cGMP production in human coronary by-pass grafts in response to stimulation with natriuretic peptides and a nitric oxide donor. Clin Sci (Lond) 1 September 2006; 111 (3): 225–231. doi: https://doi.org/10.1042/CS20060034
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