IR (ischaemia/reperfusion) injury of the intestine occurs commonly during abdominal surgery. We have previously shown that PDTC (pyrrolidine dithiocarbamate), an HO-1 (haem oxygenase-1) donor, improves intestinal microvascular perfusion. In the present study, we have investigated the effects of PDTC on the intestinal microcirculation following IR (ischaemia/reperfusion) injury of the intestine. Male Sprague–Dawley rats (n=72) were randomly assigned to four groups (n=18/group): (i) sham-operated group, who underwent laparotomy without induction of IR of the intestine; (ii) IR group, who were subjected to 30 min of superior mesenteric artery occlusion and 2 h of reperfusion; (iii) PDTC+IR group, who received PDTC prior to IR; and (iv) ZnPP group, who received the HO-1 inhibitor ZnPP (zinc protoporphyrin) followed by procedures as in group (iii). The ileum was evaluated for changes in tissue cytochrome c oxidase redox status, RBC (red blood cell) dynamics and leucocyte–endothelial interactions. The expression of HO-1 in the ileal tissue was examined at the end of the reperfusion. PDTC significantly improved the intestinal tissue oxygenation, mucosal perfusion index and RBC velocity compared with the IR and ZnPP groups. PDTC also decreased the leucocyte–endothelial interactions (P<0.05 compared with the IR and ZnPP groups). PDTC induced the expression of HO-1, whereas ZnPP abolished this effect.
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Research Article|
November 14 2006
Pyrrolidine dithiocarbamate protects the small bowel from warm ischaemia/reperfusion injury of the intestine: the role of haem oxygenase
Ismail H. Mallick;
Ismail H. Mallick
*Gastrointestinal and Hepatobiliary Research Unit, Academic Division of Surgical and Interventional Sciences, University College London, Rowland Hill Street, London NW3 2PF, U.K.
†Department of Surgery, Royal Free Hampstead NHS Trust Hospital, Rowland Hill Street, London NW3 2PF, U.K.
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Marc C. Winslet;
Marc C. Winslet
*Gastrointestinal and Hepatobiliary Research Unit, Academic Division of Surgical and Interventional Sciences, University College London, Rowland Hill Street, London NW3 2PF, U.K.
†Department of Surgery, Royal Free Hampstead NHS Trust Hospital, Rowland Hill Street, London NW3 2PF, U.K.
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Alexander M. Seifalian
*Gastrointestinal and Hepatobiliary Research Unit, Academic Division of Surgical and Interventional Sciences, University College London, Rowland Hill Street, London NW3 2PF, U.K.
Correspondence: Professor Alexander M. Seifalian (email [email protected]).
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Publisher: Portland Press Ltd
Received:
May 17 2006
Revision Received:
June 22 2006
Accepted:
August 02 2006
Accepted Manuscript online:
August 02 2006
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2006
Clin Sci (Lond) (2006) 111 (6): 373–380.
Article history
Received:
May 17 2006
Revision Received:
June 22 2006
Accepted:
August 02 2006
Accepted Manuscript online:
August 02 2006
Citation
Ismail H. Mallick, Marc C. Winslet, Alexander M. Seifalian; Pyrrolidine dithiocarbamate protects the small bowel from warm ischaemia/reperfusion injury of the intestine: the role of haem oxygenase. Clin Sci (Lond) 1 December 2006; 111 (6): 373–380. doi: https://doi.org/10.1042/CS20060119
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